Dengue virus-infected human dendritic cells reveal hierarchies of naturally expressed novel NS3 CD8 T cell epitopes.
Dengue virus-infected human dendritic cells reveal hierarchies of naturally expressed novel NS3 CD8 T cell epitopes.
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感染登革热病毒的人类树突状细胞揭示了自然表达的新型 NS3 CD8 T 细胞表位的层次结构。
DOI：
10.1111/cei.12373
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发表时间：
2014
影响因子：
4.6
通讯作者：
Rinaldo,CR
中科院分区：
文献类型：
--
作者：
Piazza,P;Campbell,D;Marques,E;Hildebrand,WH;Buchli,R;Mailliard,R;Rinaldo,CR
登革病毒（DENV）细胞介导的免疫的详细知识是有限的。在这项研究中，我们的特点CD 8 +T淋巴细胞识别三个新的和两个已知的非结构蛋白3肽表位在登革病毒感染的树突状细胞。其中3个表位的保守性较高（75-100%），而其它表位的保守性较低（0-50%）。基于抗原特异性应答的大小和多功能性的层次结构排序显示，优势表位高度保守，并且对多种DENV血清型具有交叉反应性。这些结果与DENV的发病机制和疫苗设计有关。
Detailed knowledge of dengue virus (DENV) cell-mediated immunity is limited. In this study we characterize CD8+T lymphocytes recognizing three novel and two known non-structural protein 3 peptide epitopes in DENV-infected dendritic cells. Three epitopes displayed high conservation (75–100%), compared to the others (0–50%). A hierarchy ranking based on magnitude and polyfunctionality of the antigen-specific response showed that dominant epitopes were both highly conserved and cross-reactive against multiple DENV serotypes. These results are relevant to DENV pathogenesis and vaccine design.