Genomic organization and expression of CD8α and CD8β genes in fugu Takifugu rubripes
Genomic organization and expression of CD8α and CD8β genes in fugu Takifugu rubripes
复制标题
DOI：
10.1016/j.fsi.2007.05.005
复制
发表时间：
2007-11-01
影响因子：
4.7
通讯作者：
Suzuki, Yuzuru
中科院分区：
文献类型：
--
作者：
Suetake, Hiroaki;Araki, Kyosuke;Suzuki, Yuzuru
我们已经克隆了编码α和β链的CD 8从虎河豚（河豚），红鳍东方鲀。cDNA序列编码推定的前导肽、细胞外免疫球蛋白可变区样结构域、茎区、跨膜区和胞质尾区。蛋白酪氨酸激酶p56（lck）结合基序没有发现在推定的河豚CD 8 α胞质尾。O-连接的糖基化位点被发现在两个CD 8链的茎，这表明可能的茎形成。系统发育分析表明，河豚的CD 8 α和CD 8 β链分别与其他脊椎动物的CD 8 α和CD 8 β链聚簇。河豚CD 8基因由六个外显子组成，外显子之间有五个内含子。这些基因串联排列，相距3.6 kb，并且处于相同的转录方向。定量RT-PCR分析表明，CD 8主要表达于淋巴组织。原位杂交显示河豚CD 8基因在淋巴器官内的胸腺细胞和淋巴细胞中表达。鱼类中CD 8的分子特征为开发特异性抗体以鉴定T细胞亚群提供了基础，也为深入了解CD 8的进化和适应性免疫提供了潜在的重要见解。(C)2007爱思唯尔有限公司保留所有权利。
We have cloned cDNAs encoding the alpha and beta chains of CD8 from the tiger pufferfish (fugu), Takifugu rubripes. The cDNA sequences encode a putative leader peptide, extracellular immunoglobulin variable region-like domain, stalk region, transmembrane region, and cytoplasmic tail. A protein tyrosine kinase p56(lck) binding motif was not found in the putative fugu CD8 alpha cytoplasmic tail. O-linked glycosylation sites were found in the stalk of both CD8 chains, suggesting possible stalk formation. Phylogenetic analysis showed that fugu CD8 alpha and CD8 beta chains cluster with other vertebrate CD8 alpha and CD8 beta chains, respectively. The fugu CD8 genes comprise six exons separated by five introns. The genes are tandemly aligned 3.6 kb apart and are in the same transcription orientation. Quantitative RT-PCR analysis demonstrated that fugu CD8 is expressed predominantly in lymphoid tissues. In situ hybridization showed that fugu CD8 genes are expressed in thymocytes and lymphocytes within lymphoid organs. Molecular characterization of CD8 in fish provides the basis for development of specific antibodies to identify T-cell subsets, as well as potentially important insights into the evolution of CD8 and the adaptive immunity. (C) 2007 Elsevier Ltd. All rights reserved.