Patterning to enhance activity of cultured neuronal networks.
Patterning to enhance activity of cultured neuronal networks.
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DOI：
10.1049/ip-nbt:20040706
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发表时间：
2004-06-01
期刊：
影响因子：
--
通讯作者：
Wheeler, B C
中科院分区：
文献类型：
--
作者：
Nam, Y;Chang, J;Wheeler, B C
培养胚胎大鼠海马神经元，以深入了解神经元的小网络如何相互作用。主要观察结果是用电极阵列记录的电活动，主要是自发和诱发的动作电位。几种光刻技术被开发用于以微米精度控制表面分子的图案，以控制神经元的附着和生长。使用针对蛋白质驱避剂的亲细胞聚赖氨酸，因此使用疏细胞聚乙二醇。通过将细胞平版印刷术与微电极阵列相结合，有可能将神经元优先引导到电极，并开始研究神经元网络的几何图案是否影响其神经电活动的图案。很明显，这些技术足以确保神经元与电极的接触，但不能确保记录信号，即使神经元直接位于电极顶部。神经电活动的成熟取决于培养物内神经胶质的生长，使得当神经胶质的数量与神经元的数量大致相同时，自发活动似乎变得稳健。
Embryonic rat hippocampal neurons were cultured in order to gain insights into how small networks of neurons interact. The principal observations are the electrical activities recorded with the electrode arrays, primarily action potentials both spontaneous and evoked. Several lithographic techniques were developed for controlling with micrometer precision the patterns of surface molecules in order to control neuronal attachment and growth. Cytophilic polylysine against protein repellent and hence cytophobic polyethylene glycol were used. By combining the cellular lithography with the microelectrode arrays it was possible to guide neurons preferentially to electrodes and to begin to investigate the question as to whether the geometric pattern of a neuronal network influences the patterns of its neuroelectric activity. It is clear that the techniques are adequate to ensure contact of neurons to electrodes but not to ensure the recording of signals, even when neurons lie directly on top of electrodes. The maturation of neuroelectric activity depends on the growth of glia within the culture, such that spontaneous activity appears to become robust when the number of glia is roughly the same as the number of neurons.