Relation between RLP-triglyceride to RLP-cholesterol ratio and particle size distribution in RLP-cholesterol profiles by HPLC.
Relation between RLP-triglyceride to RLP-cholesterol ratio and particle size distribution in RLP-cholesterol profiles by HPLC.
复制标题
RLP-甘油三酯与 RLP-胆固醇比率和 HPLC 测定的 RLP-胆固醇分布中的粒度分布之间的关系。
DOI：
10.1016/s0009-8981(00)00213-8
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
K. Nakajima
中科院分区：
文献类型：
--
作者：
M. Okazaki;S. Usui;N. Tada;T. Nakano;K. Nakajima
用免疫分离方法分离的残体样颗粒（RLP）在物理和生化性质上具有异质性。本研究的目的是通过高效液相色谱法研究高脂蛋白血症患者RLP-C谱中rlp -甘油三酯（RLP-TG）与rlp -胆固醇（RLP-C）比值和粒径分布之间的关系。从147例受试者血清中分离RLP。分别用酶法测定RLP-C和RLP-TG。采用高效液相色谱法测定RLP的粒径，采用4根连接的TSKgel LipopropakXL柱。基于个体RLP-C的HPLC图谱，将其分为三种不同类型：以LDL为主，以VLDL为主，以VLDL为主。所有III型高脂血症患者均以VLDL型为主，但VLDL粒径较小（32 nm）。重度高甘油三酯血症（TG>4.52 mmol−1）受试者多为VLDL型，粒径大（41 nm）。在所有非低密度脂蛋白为主的被试（n=105）中，RLP颗粒大小与RLP-TG / RLP- c比值有显著相关性（r=0.432， P<0.001），而血清TG / RLP- c比值无显著相关性（r=0.062）。提示RLP-TG与RLP-C比值可用于区分致动脉粥样硬化小颗粒脂蛋白与大颗粒富tg残余脂蛋白。
Remnant-Like Particles (RLP) isolated by an immunoseparation method are heterogeneous in their physical and biochemical properties. The objective of this study was to examine the relation between RLP-triglyceride (RLP-TG) to RLP-cholesterol (RLP-C) ratio and particle size distribution in RLP-C profiles from patients with hyperlipoproteinemia by HPLC. RLP were isolated from serum samples from 147 subjects. RLP-C and RLP-TG were quantified by respective enzymatic methods. Particle sizes of the RLP were measured using HPLC with 4 connected TSKgel LipopropakXL columns. Based on HPLC profiles of RLP-C from individual subjects, three different types were classified: predominantly LDL, predominantly VLDL, and mostly VLDL types. All patients with type III hyperlipidemia were mostly VLDL type but with smaller particle size of VLDL (32 nm) than other subjects. Severe hypertriglyceridemic (TG>4.52 mmoll−1) subjects were mostly VLDL type with large particle size (41 nm). As for all subjects (n=105) without predominantly LDL type, a significant correlation between RLP particle size and RLP-TG to RLP-C ratio (r=0.432, P<0.001) was obtained, but not in case of serum TG to RLP-C ratio (r=0.062). It suggests that RLP-TG to RLP-C ratio might be used for discrimination of atherogenic smaller-sized lipoprotein from larger-sized TG-rich lipoprotein remnants.
使用载脂蛋白 B-100 单克隆抗体从人血浆中分离出富含甘油三酯的脂蛋白的载脂蛋白 E 富集组分的特性。
影响因子：
6.5
作者：
Campos,E;Nakajima,K;Tanaka,A;Havel,RJ
通讯作者：
Havel,RJ
残余脂蛋白胆固醇和甘油三酯参考范围来自弗雷明汉心脏研究。
影响因子：
9.3
作者：
McNamara,JR;Shah,PK;Nakajima,K;Cupples,LA;Wilson,PW;Ordovas,JM;Schaefer,EJ
通讯作者：
Schaefer,EJ