Pyrethroid tolerance is associated with elevated expression of antioxidants and agricultural practice in Anopheles arabiensis sampled from an area of cotton fields in Northern Cameroon
Pyrethroid tolerance is associated with elevated expression of antioxidants and agricultural practice in Anopheles arabiensis sampled from an area of cotton fields in Northern Cameroon
复制标题
DOI：
10.1111/j.1365-294x.2007.03617.x
复制
发表时间：
2008-02-01
影响因子：
4.9
通讯作者：
Ranson, Hilary
中科院分区：
文献类型：
--
作者：
Mueller, Pie;Chouaibou, Mouhamadou;Ranson, Hilary
用杀虫剂喷洒农作物可以选择非目标昆虫的抗性，这可能会影响杀虫剂用于控制媒介传播疾病的使用。在对棉花作物施用杀虫剂的 4 个月期间之前和中间，在喀麦隆北部棉花种植区的田间群体中测定了疟疾媒介阿拉伯按蚊对溴氰菊酯的耐受性。观察到中位敲低时间增加了 1.6 倍。为了确定这种耐受性的增加是否与通常与杀虫剂抗性相关的基因的组成性升高有关，从田间捕获的蚊子家族系的 F-1 后代中提取了 RNA，并与冈比亚按蚊解毒芯片杂交。实验设计避免了定植的混杂效应，这项研究是第一个测量野生捕获的怀孕蚊子后代基因表达的研究。一些具有抗氧化作用的基因，包括超氧化物歧化酶、谷胱甘肽 S-转移酶和硫氧还蛋白依赖性过氧化物酶以及细胞色素 P450，在杀虫剂喷洒计划期间收集的蚊子家族中表现出较高的表达。这些基因可能构成针对杀虫剂的重要的一般防御机制。有趣的是，这些基因的表达水平密切相关，表明存在共同的调节机制。
Spraying of agricultural crops with insecticides can select for resistance in nontarget insects and this may compromise the use of insecticides for the control of vector-borne diseases. The tolerance of the malaria vector, Anopheles arabiensis to deltamethrin was determined in a field population from a cotton-growing region of Northern Cameroon both prior to and midway through the 4-month period of insecticide application to the cotton crop. A 1.6-fold increase in the median knockdown time was observed. To determine whether this increased tolerance was associated with constitutively elevated levels of genes commonly associated with insecticide resistance, RNA was extracted from F-1 progeny from family lines of field-caught mosquitoes and hybridized to the Anopheles gambiae detox chip. The experimental design avoided the confounding effects of colonization, and this study is the first to measure gene expression in the progeny of gravid, wild-caught mosquitoes. Several genes with antioxidant roles, including superoxide dismutases, a glutathione S-transferase and a thioredoxin-dependent peroxidase, and a cytochrome P450 showed elevated expression in mosquito families collected during the insecticide-spraying programme. These genes may constitute an important general defence mechanism against insecticides. Intriguingly, the levels of expression of these genes were strongly correlated suggesting a common regulatory mechanism.