Evidence for cycling of aggregate-containing tubules in toad urinary bladder.
Evidence for cycling of aggregate-containing tubules in toad urinary bladder.
复制标题
蟾蜍膀胱中含有聚集物的小管循环的证据。
DOI：
10.1111/j.1768-322x.1985.tb00428.x
复制
发表时间：
1985
影响因子：
2.7
通讯作者：
Hays,RM
中科院分区：
文献类型：
--
作者：
Ding,G;Franki,N;Hays,RM
抗利尿激素(ADH)促进胞浆管状结构与受体组织(如蟾蜍膀胱)的管腔膜融合。为了确定融合是否是一个连续的循环过程，在腔内洗浴介质中用含胶体金的ADH刺激膀胱。在短短15分钟的刺激后，在细胞质中可以看到充满金色的小管，这表明确实发生了循环。连续切片证实，这些小管与管腔膜无连接，已返回细胞质。停止ADH刺激，然后进行第二次刺激，极大地减少了充满金的细胞质小管的数量，表明许多小管能够再融合。平均融合事件直径发生显著变化，在15min时增大，60min时缩小。因此，ADH启动了细胞质小管在细胞质和管腔膜之间的连续循环过程。
Antidiuretic hormone (ADH) promotes the fusion of cytoplasmic tubular structures with the luminal membrane of receptor tissues such as toad urinary bladder. To determine whether fusion is a continuous cyclic process, bladders were stimulated with ADH with colloidal gold in the luminal bathing medium. After as little as 15 min of stimulation, gold‐filled tubules were seen in the cytoplasm, evidence that cycling was indeed taking place. Serial sections confirmed that these tubules had no connection with the luminal membrane, and had returned to the cytoplasm. Cessation of ADH stimulation, followed by a second stimulation, greatly reduced the number of gold‐filled cytoplasmic tubules, suggesting that many tubules were capable of refusion. Mean fusion event diameter underwent significant changes, enlarging at 15 min, and contracting at 60 min. Thus, ADH initiates a process of continuous cycling of cytoplasmic tubules between cytoplasm and luminal membrane.