CHARACTERIZATION OF INTERMEDIATE (10 NM) FILAMENTS OF CULTURED-CELLS USING AN AUTO-IMMUNE RABBIT ANTISERUM
CHARACTERIZATION OF INTERMEDIATE (10 NM) FILAMENTS OF CULTURED-CELLS USING AN AUTO-IMMUNE RABBIT ANTISERUM
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DOI：
10.1016/0092-8674(78)90194-0
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
BURRIDGE, K
中科院分区：
文献类型：
--
作者：
GORDON, WE;BUSHNELL, A;BURRIDGE, K
在未免疫的家兔中发现了一种抗血清，它与各种成纤维细胞、上皮细胞、巨噬细胞和神经母细胞瘤中的细丝系统发生强烈反应。这些细丝与肌动蛋白抗体所见的肌动蛋白微丝束不同，并且它们不受细胞松弛素B短暂处理的影响。这些细丝的模式有点类似于微管的描述，但通过比较细胞分裂期间各自的免疫荧光模式，可以将细丝与微管清楚地区分开来。在响应药物秋水仙胺和长春碱，与此preimmune血清反应的细丝凝聚形成一个紧凑的核周线圈的纤维，分布和行为与先前描述的10 nm或中间丝的电子显微镜研究。支持我们的结论，这种抗血清与中间丝反应的进一步证据是通过比较电子显微镜和免疫荧光模式从平行的细胞培养。
An antiserum has been found in a nonimmunized rabbit which reacts strongly with a system of filaments in various fibroblasts, epithelial cells, macrophages and neuroblastoma. These filaments are distinct from the actin microfilament bundles visualized by an antibody against actin, and they are not affected by brief treatment with cytochalasin B. The pattern of these filaments somewhat resembles that described for microtubules, but the filaments could be clearly distinguished from microtubules by a comparison of their respective immunofluorescent patterns during cell division. In response to the drugs colcemid and vinblastine, the filaments reacting with this preimmune serum condense to form a compact perinuclear coil of fibers, a distribution and behavior in agreement with that previously described for the 10 nm or intermediate filaments studied by electron microscopy. Further evidence supporting our conclusion that this antiserum reacts with intermediate filaments is provided by a comparison of electron micrographs and the immunofluorescent patterns from parallel cell cultures.