WNT-5A AND WNT-7A ARE EXPRESSED IN THE DEVELOPING CHICK LIMB BUD IN A MANNER SUGGESTING ROLES IN PATTERN-FORMATION ALONG THE PROXIMODISTAL AND DORSOVENTRAL AXES
WNT-5A AND WNT-7A ARE EXPRESSED IN THE DEVELOPING CHICK LIMB BUD IN A MANNER SUGGESTING ROLES IN PATTERN-FORMATION ALONG THE PROXIMODISTAL AND DORSOVENTRAL AXES
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DOI：
10.1016/0925-4773(93)90034-u
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发表时间：
1993-10-01
影响因子：
2.6
通讯作者：
KOSHER, RA
中科院分区：
文献类型：
--
作者：
DEALY, CN;ROTH, A;KOSHER, RA
Wnt基因家族编码一组分泌的信号分子，这些分子参与胚胎发生过程中细胞命运和模式形成的调节。我们研究了鸡Wnt家族的两个成员，Wnt-5a和Wnt-7a，在鸡肢芽发育过程中的表达模式。Wnt-5a在指导肢中胚层向外生长的顶端外胚层嵴中表达。Wnt-5a还表现出三个定量不同的域的表达沿着proximodistal（PD）轴的肢体中胚层，可能对应的区域，这将产生三个不同的PD段的肢体，autopod，zeugopod，和stylopod。相反，Wnt-7a在肢芽中的表达特别限于背外胚层。这些观察结果表明，Wnt-5a和Wnt-7a在图案形成沿着PD和背腹轴的发展鸡肢芽可能的作用。此外，Wnt-5a和Wnt-7a在鸡胚的几个其他区域中表现出空间离散的表达结构域，这与这些基因在各种其他组织中的发育作用一致。
The Wnt gene family encodes a group of secreted signalling molecules that have been implicated in the regulation of cell fate and pattern formation during embryogenesis. We have examined the patterns of expression of two members of the chicken Wnt family, Wnt-5a and Wnt-7a, during development of the chick limb bud. Wnt-5a is expressed in the apical ectodermal ridge which directs outgrowth of limb mesoderm. Wnt-5a also exhibits three quantitatively distinct domains of expression along the proximodistal (PD) axis of the limb mesoderm that may correspond to the regions which will give rise to the three distinct PD segments of the limb, the autopod, zeugopod, and stylopod. In contrast, Wnt-7a expression in the limb bud is specifically limited to the dorsal ectoderm. These observations suggest possible roles for Wnt-5a and Wnt-7a in pattern formation along the PD and dorsoventral axes of the developing chick limb bud. In addition, Wnt-5a and Wnt-7a exhibit spatially discrete domains of expression in several other regions of the chick embryo consistent with developmental roles for these genes in a variety of other tissues.