Recognition of Pneumocystis carinii antigens by local antibody-secreting cells following resolution of P-carinii pneumonia in mice
Recognition of Pneumocystis carinii antigens by local antibody-secreting cells following resolution of P-carinii pneumonia in mice
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DOI：
10.1086/515607
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发表时间：
1998-07-01
影响因子：
6.4
通讯作者：
Harmsen, AG
中科院分区：
文献类型：
--
作者：
Gigliotti, F;Garvy, BA;Harmsen, AG
为了检测卡氏肺囊虫抗原库，在卡氏肺炎康复后立即从气管支气管淋巴结分离抗体分泌B细胞识别卡氏肺囊虫抗原，从这些细胞中产生单克隆抗体（mab）。与以往的系统性免疫研究相反，卡氏P - gpA不是这些B细胞识别的免疫优势抗原。54份P， carinii特异性杂交瘤培养上清液中有49份（91%）与P， carinii抗原反应，而不是与gpA反应。许多由此产生的单克隆抗体识别了在囊肿和滋养体表面表达的以前未表征的抗原。使用克隆的单克隆抗体之一进行Western blotting，发现与广泛的抗原物质具有反应性，在印迹的50至65 kda区域具有最强烈的反应性。这些单克隆抗体鉴定出的抗原值得进一步研究，因为它们在I？囊虫肺炎。
To examine the repertoire of Pneumocystis carinii antigens recognized by antibody-secreting B cells from tracheobronchial lymph nodes isolated immediately following recovery from P, carinii pneumonia, monoclonal antibodies (MAbs) were produced from these cells. In contrast to previous studies of systemic immunity, P, carinii gpA was not the immunodominant antigen recognized by these B cells. Forty-nine (91%) of 54 P, carinii-specific hybridoma culture supernatants reacted with P, carinii antigens other than gpA. Many of the resulting MAbs recognized a previously uncharacterized antigen expressed on the surface of both cysts and trophozoites. Western blotting using one of the cloned MAbs revealed reactivity with a broad range of antigenic material, with the most intense reactivity in the 50- to 65-kDa region of the blot. The antigens identified by these MAbs merit further investigation regarding protective immunity to P. carinii because they were recognized by B cells in the context of recovery from I? carinii pneumonia.