Hyperglycemia can cause membrane lipid peroxidation and osmotic fragility in human red blood cells.
Hyperglycemia can cause membrane lipid peroxidation and osmotic fragility in human red blood cells.
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DOI：
10.1016/s0021-9258(19)30085-7
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发表时间：
1989-12
期刊：
影响因子：
--
通讯作者：
S. Jain
中科院分区：
文献类型：
--
作者：
S. Jain
本文研究了葡萄糖浓度升高对人红细胞膜脂质过氧化和渗透脆性的影响。在37 °C下，将脱乙酰基的全血或RBC与不同浓度的葡萄糖孵育24小时。与对照红细胞相比，高浓度葡萄糖孵育的红细胞膜脂质过氧化反应显着增加。葡萄糖诱导的膜脂质过氧化程度与红细胞渗透脆性呈显著正相关。葡萄糖诱导的膜脂质过氧化和渗透脆性时，红细胞预处理氟，葡萄糖代谢的抑制剂;与维生素E，抗氧化剂;与对氯汞苯甲酸和甲吡酮，细胞色素P-450系统的抑制剂;或与二甲基呋喃，二苯胺，硫脲，氧自由基的清除剂。用升高的葡萄糖浓度处理的RBC也显示NADPH水平增加。外源性添加NADPH正常红细胞裂解物诱导膜脂质过氧化反应中观察到的葡萄糖处理的红细胞相似。这些数据表明，葡萄糖水平升高可引起人红细胞膜脂质过氧化反应。
The present study has examined the effect of elevated glucose levels on membrane lipid peroxidation and osmotic fragility in human red blood cells (RBC). Defibrinated whole blood or RBC were incubated with varying concentrations of glucose at 37 °C for 24 h. RBC incubated with elevated levels of glucose showed a significantly increased membrane lipid peroxidation when compared with control RBC. A significant positive correlation was observed between the extent of glucose-induced membrane lipid peroxidation and the osmotic fragility of treated RBC. Glucose-induced membrane lipid peroxidation and osmotic fragility were blocked when RBC were pretreated with fluoride, an inhibitor of glucose metabolism; with vitamin E, an antioxidant; withpara-chloromercurobenzoate and metyrapone, inhibitors of the cytochrome P-450 system; or with dimethylfurane, diphenylamine, and thiourea, scavengers of oxygen radicals. RBC treated with elevated glucose concentrations also showed an increase in NADPH levels. Exogenous addition of NADPH to normal RBC lysate induced membrane lipid peroxidation similar to that observed in the glucose-treated RBC. These data suggest that elevated glucose levels can cause the peroxidation of membrane lipids in human RBC.