Genetic analysis of betanodaviruses in subclinically infected aquarium fish and invertebrates
Genetic analysis of betanodaviruses in subclinically infected aquarium fish and invertebrates
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DOI：
10.1007/s00284-008-9116-x
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发表时间：
2008-05-01
影响因子：
2.6
通讯作者：
Park, Se Chang
中科院分区：
文献类型：
--
作者：
Gomez, Dennis K.;Baeck, Gun Wook;Park, Se Chang
引起病毒性神经坏死（VNN）的贝塔诺达病毒已在世界范围内的几种养殖海洋鱼类中检测到并分离到。在韩国，VNN在几种养殖海鱼中被鉴定。本研究提供了来自韩国一家私人商业水族馆收集的不同物种的表面健康的水族馆鱼类和无脊椎动物的11种野田病毒株的扩增巢式PCR产物（420 bp）的数据。基于RNA 2外壳蛋白基因部分核苷酸序列（177个碱基）的系统发育分析与红斑石斑鱼神经坏死病毒（RGNNV）基因型一致（96%~ 100%）。在这些从不同国家进口的亚临床感染的观赏鱼和无脊椎动物中存在RGNNV型贝塔诺达病毒，可能表明样品在水族馆内受到污染，这对管理病毒性神经坏死提出了严重挑战。这些阳性样本可能是贝他诺病毒感染水族馆内其他易感鱼类的接种源。
Betanodaviruses causing viral nervous necrosis (VNN) have been detected and isolated from several species of cultured marine fish worldwide. In Korea, VNN was identified in several species of cultured marine fish. This study presents data on the amplified nested PCR product (420 bp) of 11 nodavirus strains from different species of apparently healthy aquarium fish and invertebrates collected in one private commercial aquarium in Korea. Phylogenetic analyses based on the partial nucleotide sequence (177 bases) of the RNA2 coat protein gene were identical to the redspotted grouper nervous necrosis virus (RGNNV) genotype (96%-100%). The presence of the RGNNV type of betanodaviruses in these subclinically infected aquarium fish and invertebrates imported from different countries probably indicates that the samples were contaminated inside the aquarium and represents a serious challenge for its management of viral nervous necrosis. These positive samples can be an inoculum source of betanodavirus infection to other susceptible fish species inside the aquarium.