Search for the evolutionary origin of a brain:: Planarian brain characterized by microarray
Search for the evolutionary origin of a brain:: Planarian brain characterized by microarray
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DOI：
10.1093/molbev/msg086
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发表时间：
2003-05-01
影响因子：
10.7
通讯作者：
Gojobori, T
中科院分区：
文献类型：
--
作者：
Nakazawa, M;Cebrià, F;Gojobori, T
大脑的起源仍然是进化研究中的一个具有挑战性的问题。为了了解结构性大脑何时以及如何出现，我们分析了低等无脊椎动物涡虫的中枢神经系统（CNS）。我们通过构建cDNA微阵列对涡虫头部特异性基因进行了大规模筛选。涡虫头部和身体其他部分之间的 cDNA 竞争性杂交揭示了 205 个带有头部特异性尖峰的基因，其中包括脊椎动物神经系统中的必需基因。前 30 个基因的表达模式显示出最强的尖峰，这表明涡虫大脑已经经历了功能区域化。我们展示了涡虫大脑的复杂细胞结构，尽管其形态简单、肤浅。
The origin of the brain remains a challenging problem in evolutionary studies. To understand when and how the structural brain emerged, we analyzed the central nervous system (CNS) of a lower invertebrate, planarian. We conducted a large-scale screening of the head part-specific genes in the planarian by constructing a cDNA microarray. Competitive hybridization of cDNAs between a head portion and the other body portion of planarians revealed 205 genes with head part-specific spikes, including essential genes in the vertebrate nervous system. The expression patterns of the top 30 genes showing the strongest spikes implied that the planarian brain has undergone functional regionalization. We demonstrate the complex cytoarchitecture of the planarian brain, despite its simple superficiality of the morphology.