Spectroscopic Investigation of Increased Fluorescent Intensity of Fluorescent Dyes When Adsorbed onto Polystyrene Microparticles
Spectroscopic Investigation of Increased Fluorescent Intensity of Fluorescent Dyes When Adsorbed onto Polystyrene Microparticles
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DOI：
10.2116/analsci.20scp22
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发表时间：
2021-05-01
影响因子：
1.6
通讯作者：
Habe, Hiroshi
中科院分区：
文献类型：
--
作者：
Aoki, Hiroshi;Torimura, Masaki;Habe, Hiroshi
作为环境污染物的微塑料越来越引起人们的警觉。微塑料的特性将是必要的，以区分微塑料从其他类型的颗粒。为了区分特定的微塑料，使用塑料可吸附的荧光染料，通过过滤将染色的微塑料从染料-微塑料混合物中分离出来，并通过荧光显微镜分析微塑料的荧光染色类型。本研究为实现荧光染色的原位分析，为了在没有任何分离或过滤过程的情况下区分微塑料，我们使用3D激发发射矩阵荧光光谱（激发波长依赖发射光谱）研究了荧光染料吸附到微塑料颗粒表面后荧光性质的变化。我们使用三种荧光染料：黄绿素、罗丹明6 G和亚甲蓝，以及聚苯乙烯微粒作为我们的模型微塑料。黄绿素和亚甲蓝显示荧光强度增加，而罗丹明6 G显示可忽略的强度变化。这可能是由于染料对聚苯乙烯颗粒表面的亲和力程度、表面上染料的结构稳定性以及每种染料吸附到表面后染料周围环境的变化。我们的结论是，我们已经证明了寻找合适的荧光染料使用这里研究的方法来识别和估计个别塑料材料的潜力。
Microplastics as environmental pollutants are increasingly a source of alarm. The characterization of microplastics will be necessary to discriminate microplastics from other types of particles. To discriminate specific microplastics, plastic-adsorbable fluorescent dyes are used, the stained microplastics are separated from the dye-microplastic mixture by filtration, and the type of fluorescent staining of the microplastics is analyzed by fluorescent microscopy. In this study, to realize the in situ analysis of fluorescent staining, i.e., to discriminate microplastics without any separation or filtration processes, we studied the change in the fluorescent properties after adsorption of the fluorescent dyes to the microplastic particle surfaces using a 3D excitation emission matrix fluorescence spectroscopy (the excitation wavelength-dependent emission spectrum). We used three fluorescent dyes: Fluorescein, Rhodamine 6G, and Methylene Blue, and polystyrene microparticles as our model microplastic. Fluorescein and Methylene Blue showed increases in the fluorescent intensity, while Rhodamine 6G showed negligible intensity changes. This is likely due to the degree of affinity of the dyes to the polystyrene particle surface, the structural stability of the dyes on the surface, and the changes in the environment around the dyes after the adsorption of each dye to the surface. We conclude that we have demonstrated the potential to look for appropriate fluorescent dyes using the method studied here to identify and estimate individual plastic materials.