Low-threshold neuronal activity of spinal dorsal horn neurons increases during REM sleep in cats: comparison with effects of anesthesia.
Low-threshold neuronal activity of spinal dorsal horn neurons increases during REM sleep in cats: comparison with effects of anesthesia.
复制标题
猫快速眼动睡眠期间脊髓背角神经元的低阈值神经元活动增加：与麻醉效果的比较。
DOI：
10.1152/jn.1995.74.2.763
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Collins,JG
中科院分区：
文献类型：
--
作者：
Kishikawa,K;Uchida,H;Yamamori,Y;Collins,JG
1.制作猫脊髓背角腰部膨大的慢性记录。在每次记录开始时，一个钨微电极穿过生理完好、清醒、没有药物的动物的硬脑膜，直到幅度辨别为单个神经元提供后腿上的感受场。2.在触觉和热感、非伤害性和伤害性刺激下，记录各神经元的细胞外活动。3.将清醒状态下获得的基线反应与相同神经元在慢波或快眼动(REM)睡眠时的反应进行比较。在睡眠研究结束后，观察麻醉(异丙酚7.5 mg/kg iv)对神经元亚群的影响。4.所研究的七个神经元在快速眼动睡眠中的低阈值感受野均较基础值增大。平均增长52.6%(26.2~96.7%)。5.异丙酚麻醉可使REM睡眠中7个神经元的低阈值感受野平均减少49.1%(范围29-74%)。6.在REM睡眠中，观察到15个神经元对感受野刷检的反应。观察异丙酚对其中8个神经元感受野刷屏的影响。在这八个神经元中，只有一个神经元受到快速眼动睡眠和麻醉的影响是相同的。7.慢波睡眠中神经元反应的变化不太一致，但仍不同于异丙酚引起的变化。
1. Cats were prepared for chronic recordings from the lumbar enlargement of the spinal dorsal horn. At the beginning of each recording session, a tungsten microelectrode was advanced through the dura in a physiologically intact, awake, drug-free animal, until amplitude discrimination provided a single neuron with a receptive field on the hindquarters. 2. Extracellular recordings of activity of each neuron were made during receptive field stimulation with tactile and thermal nonnoxious and noxious stimuli. 3. Baseline responses obtained in the awake state were compared with responses of the same neurons during slow-wave or rapid-eye-movement (REM) sleep. In a subpopulation of neurons, the effects of anesthesia (propofol, 7.5 mg/kg iv) were observed after the completion of sleep studies. 4. The low-threshold receptive fields of the seven neurons studied during REM sleep were all increased in size when compared with the baseline value. The average increase was 52.6% (range 26.2–96.7%). 5. The low-threshold receptive fields of the seven neurons studied during REM sleep were reduced by propofol anesthesia by an average of 49.1% (range 29–74%). 6. Neuronal response to receptive field brushing was observed in 15 neurons during REM sleep. The effect of propofol on receptive field brushing was observed in 8 of those neurons. In only one of those eight neurons were the effects of REM sleep and anesthesia in the same direction. 7. Changes in neuronal responses were less consistent during slow-wave sleep but still differed from changes induced by propofol.(ABSTRACT TRUNCATED AT 250 WORDS)