pH-Neutralization, Redox-Balanced Process with Coupled Formate Dehydrogenase and Glucose Dehydrogenase Supports Efficient Xylitol Production in Pure Water
pH-Neutralization, Redox-Balanced Process with Coupled Formate Dehydrogenase and Glucose Dehydrogenase Supports Efficient Xylitol Production in Pure Water
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pH 中和、氧化还原平衡工艺，结合甲酸脱氢酶和葡萄糖脱氢酶，支持纯水中木糖醇的高效生产
DOI：
10.1021/acs.jafc.9b05626
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发表时间：
2020
影响因子：
6.1
通讯作者：
Chen Yong
中科院分区：
文献类型：
--
作者：
Zhang Di;Chang Ziyue;Li Nan;Lei Ming;Wang Zhenyu;Niu Huanqing;Gao Nan;Liu Dong;Chen Yong
酶法生产木糖醇是一种很有前途的替代化学氢化工艺的方法。然而，它遇到的问题主要是由于蛋白质对环境因素的敏感性。本研究为了开发一种稳健、实用的木糖醇酶法生产工艺，构建了由甲酸脱氢酶（FDH）、葡萄糖脱氢酶（GDH）和木糖还原酶（XR）组成的偶联酶系统，其中FDH产生的碱性产物和酸性产物GDH产生的产物可以在辅因子再生过程中相互中和。经过条件优化，pH中和，氧化还原平衡的过程，可以在纯水中进行，不需要pH调节。结果表明，24 h内从2 M木糖获得的木糖醇产量为273.6g/L，远高于目前报道的那些，具有相对较高的生产率11.4g/（L·h）。这里展示的策略可以适用于其他NADH消耗产品的生产。
Enzymatic production of xylitol is a promising alternative to the chemical hydrogenation process. However, it encounters problems that are largely due to protein susceptibility to environmental factors. In this study, to develop a robust, practical enzymatic process for xylitol production, a coupled enzyme system consisting of formate dehydrogenase (FDH), glucose dehydrogenase (GDH), and xylose reductase (XR) was constructed, wherein the alkaline product produced by FDH and the acidic product produced by GDH could neutralize each other during cofactor regeneration. After optimization of conditions, a pH-neutralization, redox-balanced process was developed that could be carried out in pure water requiring no pH regulation. As a result, a xylitol production of 273.6 g/L that is much higher than those yet reported was obtained from 2 M xylose in 24 h, with a relatively high productivity of 11.4 g/(L h). The strategy demonstrated here can be adapted for the production of other NADH-consuming products.