PRESERVATION OF FOWL SEMEN IN LIQUID-NITROGEN - IMPROVED METHOD
PRESERVATION OF FOWL SEMEN IN LIQUID-NITROGEN - IMPROVED METHOD
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DOI：
10.1080/00071667808416462
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发表时间：
1978-01-01
影响因子：
2
通讯作者：
STEWART, JM
中科院分区：
文献类型：
--
作者：
LAKE, PE;STEWART, JM
本文介绍了一种改进的鸡精液液氮保存方法。它使用甘油作为冷冻保护剂，精液储存长达两年半的受精率是非常有希望的。该方法的基本特点是冻结速度慢，温度降低到-35度。C，然后将精液放入液态N中，并在解冻并从稀释液中去除甘油后15分钟内授精。通过更好地控制液氮注入冷冻室，从而最大限度地减少冷冻过程中安瓶周围局部、快速的温度变化，有望进一步改进该技术。初步结果表明，根据精子耐受冷冻的能力来选择男性可能是可能的。讨论了在确定储存精液的受精能力时，认识到男性在精液初始质量上的差异和女性在生殖器官生理上的差异的重要性。
An improved method of storing fowl semen in liquid N is described. It uses glycerol as a cryoprotectant, and the degree of fertility obtained with semen stored for up to 2 1/4 yr is very promising. The essential features of the method are a slow freezing rate, a reduction of temperature to -35.degree. C before the semen is plunged into liquid N and insemination within 15 min of thawing and removing the glycerol from the diluent. Further improvements in the technique may be expected through better control of the injection of liquid N into the freezing chamber, thereby minimizing localized, rapid temperature changes around the ampoules during freezing. Preliminary results suggest that it may prove possible to select males for the ability of their spermatozoa to withstand freezing. The importance of recognizing differences between males in the initial quality of semen and between females in the physiology of the reproductive organs in determining the fertilizing ability of stored semen is discussed.