A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics
A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics
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DOI：
10.1073/pnas.95.4.1460
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发表时间：
1998-02-17
影响因子：
11.1
通讯作者：
SantaLucia, J
中科院分区：
文献类型：
--
作者：
SantaLucia, J
提出了聚合物、哑铃和寡核苷酸最近邻（NN）热力学的统一观点，来自七个实验室的DNA NN δ G（37）度参数以相同的格式呈现，以便进行仔细的比较。这七个研究使用来自天然聚合物、合成聚合物、寡核苷酸哑铃和寡核苷酸双链的数据来推导NN参数；采用不同的数据分析方法；使用不同的盐浓度：并以不同的格式呈现了神经网络热力学，由于这些差异，关于DNA聚合物和低聚物的神经网络热力学存在很多混乱，在这里，我表明，其中六项研究实际上彼此非常一致，并且在差异仍然存在的情况下提供了解释。此外，一组参数，源自108个寡核苷酸双链，充分描述了聚合物和低聚物热力学。对于寡核苷酸和聚合物，也推导出经验盐依赖性。
A unified view of polymer, dumbbell, and oligonucleotide nearest-neighbor (NN) thermodynamics is presented, DNA NN Delta G(37)degrees parameters from seven laboratories are presented in the same format so that careful comparisons can be made, The seven studies used data from natural polymers, synthetic polymers, oligonucleotide dumbbells, and oligonucleotide duplexes to derive NN parameters; used different methods of data analysis; used different salt concentrations: and presented the NN thermodynamics in different formats, As a result of these differences, there has been much confusion regarding the NN thermodynamics of DNA polymers and oligomers, Herein I show that six of the studies are actually in remarkable agreement with one another and explanations are provided in cases where discrepancies remain, Further, a single set of parameters, derived from 108 oligonucleotide duplexes, adequately describes polymer and oligomer thermodynamics, Empirical salt dependencies are also derived for oligonucleotides and polymers.