Crucial role of peroxiredoxin III in placental antioxidant defense of mice
Crucial role of peroxiredoxin III in placental antioxidant defense of mice
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DOI：
10.1016/j.febslet.2008.05.050
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发表时间：
2008-07-09
期刊：
影响因子：
3.5
通讯作者：
Kanno, Naoko
中科院分区：
文献类型：
--
作者：
Li, Lianqin;Shoji, Wataru;Kanno, Naoko
我们观察到过氧化物酶 III (PrxIII) 基因敲除母鼠频繁死产。实时定量 PCR (qRT-PCR) 和蛋白质印迹分析显示，缺乏 PrxIII 的胎盘氧化应激增加。我们没有发现 PrxIII 敲除母鼠和野生型同窝小鼠在血液学参数、胎儿数量和胚胎发育方面存在显着差异。尽管如此，我们注意到妊娠基因敲除小鼠的红细胞中 PrxI 的表达增强。我们的结果提供了体内证据，证明 PrxIII 在胎盘抗氧化防御中发挥着至关重要的作用。 PrxI 的上调可能提供保护红细胞免受氧化损伤的补偿。 (C) 2008 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
We observed frequent stillbirth in peroxiredoxin III (PrxIII) knockout maternal mice. Quantitative real time PCR (qRT-PCR) and Western-blot analysis revealed increased oxidative stress in placentas that were deficient in PrxIII. We did not find significant difference between PrxIII knockout maternal mice and wild-type littermates in hematological parameters, fetal number, and embryonic development. Nevertheless, we noticed enhanced expression of PrxI in erythrocytes of pregnant knockout mice. Our results provided in vivo evidence that PrxIII played a crucial role in placental antioxidant defense. Up-regulation of PrxI might provide a compensation that protected erythrocytes against oxidative damage. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.