Oxytocin null mice ingest enhanced amounts of sweet solutions during light and dark cycles and during repeated shaker stress
Oxytocin null mice ingest enhanced amounts of sweet solutions during light and dark cycles and during repeated shaker stress
复制标题
DOI：
10.1016/j.bbr.2006.03.028
复制
发表时间：
2006-07-15
影响因子：
2.7
通讯作者：
Amico, Janet A.
中科院分区：
文献类型：
--
作者：
Billings, Lyndsey B.;Spero, Jonathan A.;Amico, Janet A.
中枢催产素（OT）通路似乎限制了甜溶液的摄入。与野生型（WT）小鼠相比，缺乏催产素基因的雄性和雌性C57BL/6小鼠（OT KO小鼠）在两瓶选择蔗糖溶液时，表现出初始和持续增加的蔗糖溶液摄入量[Amico JA， Vollmer RR, Cai HM, Miedlar JA, Rinaman R.]。[J].中国生物医学工程学报（英文版）；2009;33(2):591 - 591。在这项研究中，我们研究了一种非营养性甜味剂的摄入情况，即蔗糖中0.2%的糖精，OT， KO和WT小鼠给予两瓶糖精溶液和水随意选择4天。与WT小鼠相比，OT - KO小鼠在研究的第一天和随后几天的黑暗和光照期间消耗了大量的糖精溶液。在同样的动物身上重复实验，结果是相同的。在另一项实验中，我们确定每天暴露于平台振动器压力下不会改变OT - KO小鼠的显著蔗糖消耗。在应激和非应激期间，OT - KO小鼠明显比WT小鼠喝更多的蔗糖。我们得出的结论是，OT - KO小鼠对加糖溶液的狂热消费并不局限于单一的光周期，与加糖溶液的热量含量无关，并且不会因暴露于平台振动器的日常压力而改变。我们对OT KO和WT雄性和雌性小鼠摄取蔗糖和糖精的累积研究结果表明，甜味在OT神经元的募集中起着特殊的作用。(c) 2006 Elsevier B.V.版权所有
Central oxytocin (OT) pathways appear to limit consumption of sweet solutions. Male and female C57BL/6 mice that lack the gene for oxytocin (OT KO mice) displayed an initial and sustained enhanced intake of sucrose solution over water compared to wild type (WT) mice when the solutions were presented as a two-bottle choice [Amico JA, Vollmer RR, Cai HM, Miedlar JA, Rinaman R. Enhanced initial and sustained intake of sucrose solution in mice with an oxytocin gene deletion. Am J Physiol: Regul Integr Comp Physiol 2005;289:R1798-806]. In this study we examined the ingestion of a non-nutritive sweetener, 0.2% saccharin in sucrose-experienced OT KO and WT mice given a two-bottle choice between saccharin solution and water available ad libitum for 4 days. Compared to WT mice, OT KO mice consumed significantly greater volumes of saccharin solution during the dark and light photoperiods on the first day and subsequent days of the study. The results were replicated when the experiment was repeated in the same animals. In another experiment, we determined that daily exposure to platform shaker stress did not alter the marked sucrose consumption in OT KO mice. OT KO mice drank significantly more sucrose than WT mice during periods of stress and non-stress. We conclude that the avid consumption of sweetened solutions by OT KO mice is not restricted to a single photoperiod, occurs independent of caloric content of the sweetened solution, and is not altered by exposure to the daily stress of platform shaker. The cumulative results from our studies of sucrose and saccharin ingestion in OT KO and WT male and female mice suggest a special role for sweet taste in the recruitment of OT lneurons. (c) 2006 Elsevier B.V. All rights reserved.