Effect of propolis on virulence factors of Candida albicans
Effect of propolis on virulence factors of Candida albicans
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DOI：
10.1179/joc.2003.15.5.454
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发表时间：
2003-10-01
影响因子：
1.8
通讯作者：
Strippoli, V
中科院分区：
文献类型：
--
作者：
D'Auria, FD;Tecca, M;Strippoli, V
蜂胶是蜜蜂从植物中收集的一种可吸收物质。它的抗菌活性已被充分证明，但很少有人知道它对白色念珠菌毒力因子的活性。因此，这项工作的目的是在体外评估的酵母菌菌丝体转换（Y-M），细胞外磷脂酶活性和真菌粘附上皮细胞的影响。所用的两种样品对C.白色念珠菌菌株，显示出快速（30秒至15分钟）、剂量依赖性的杀细胞活性和在0.22 mg/ml浓度下对Y-M转化的抑制作用。此外，即使在较低的浓度下，菌丝长度也会减少。蜂胶还引起磷脂酶活性的剂量和时间依赖性抑制。对颊上皮细胞粘附和表面结构疏水性没有明显影响，但碘化丙啶试验证明了对质膜结构的损伤。
Propolis is a resinous substance collected by honeybees from plant sources. Its antimicrobial activity has been well documented but little is specifically known about its activity on virulence factors of Candida albicans. The aim of this work was therefore to evaluate in vitro the propolis effect on yeast-mycelial conversion (Y-M), extracellular phospholipase activity and fungal adhesion to epithelial cells. The two propolis samples used significantly inhibited the C. albicans strains tested, showing a rapid (between 30 seconds and 15 minutes), dose-dependent cytocidal activity and an inhibitory effect on Y-M conversion at a concentration of 0.22 mg/ml. Moreover, the hyphal length was reduced even at lower propolis concentration. Propolis also caused a dose- and time-dependent inhibition of phospholipase activity. No clear effect was shown on adherence to buccal epithelial cells and surface structure hydrophobicity, but damage to the plasma membrane structure was demonstrated with the Propidium Iodide test.