300,000 species to identify: problems, progress, and prospects in DNA barcoding of land plants
300,000 species to identify: problems, progress, and prospects in DNA barcoding of land plants
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DOI：
10.2307/25065638
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发表时间：
2006-08-01
期刊：
影响因子：
3.4
通讯作者：
Savolainen, Vincent
中科院分区：
文献类型：
--
作者：
Cowan, Robyn S.;Chase, Mark W.;Savolainen, Vincent
DNA条形码已经成功地应用于有限数量的动物群体，应用线粒体基因，细胞色素c氧化酶亚基1。最近两个DNA区域，质体trnH-psbA间隔区和核核糖体ITS区，已被证明有潜力作为陆地植物的识别条形码，虽然有一些显着的缺点。理想的条形码长度应该相对较短（类似于700 bp）。种间比种内更易变，并且容易用通用引物扩增。在目前成功的基础上，正在进行的调查正在寻找适用于所有陆地植物的最佳条形码。一旦建立，植物条形码可以有效地用于生物多样性清单，保护评估和应用法医调查。测序技术的进步和DNA条形码库的完成有可能为公众提供更多关于自然世界的信息。
DNA barcodes have been successfully applied to a limited number of animal groups with the application of the mitochondrial gene, cytochrome c oxidase subunit 1. Recently two DNA regions, the plastid trnH-psbA spacer and nuclear ribosomal ITS region, have been shown to have potential as an identification barcode for land plants, although with some significant drawbacks. The ideal barcode should be relatively short in length (similar to 700 bp),. more variable between than within species, and easily amplifiable with universal primers. Building on current success, ongoing investigations are searching for the best barcode to apply to all land plants. Once established, a plant barcode may be effectively used in biodiversity inventories, conservation assessments, and applied forensic investigations. Advances in sequencing technology and the completion of the DNA barcode library have the potential to provide the public with increased access to information about the natural world.