Ventricular proliferation zones in the brain of an adult teleost fish and their relation to neuromeres and migration (secondary matrix) zones
Ventricular proliferation zones in the brain of an adult teleost fish and their relation to neuromeres and migration (secondary matrix) zones
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DOI：
10.1002/cne.1056
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发表时间：
2001-07-16
影响因子：
2.5
通讯作者：
Ohlin, LM
中科院分区：
文献类型：
--
作者：
Ekström, P;Johnsson, CM;Ohlin, LM
通过放射自显影检测H-3-胸苷和免疫细胞化学检测胸苷类似物5 '-溴脱氧尿苷（BrdU）（单独或组合），以及通过单克隆抗体免疫细胞化学检测增殖细胞核抗原（PCNA），鉴定了成年三棘鱼脑中含有活跃分裂细胞的区域（增殖区：PZ）。PZs与成人大脑区域之间的边界以及定义的形态功能细分有关。PZ位于端脑和间脑之间的边界，以及中脑和菱脑之间的边界。在中脑，PZ遵循每个顶盖半球的背内侧、尾侧和腹外侧方面，在枕圆的尾侧方面延伸到瓣膜外侧核。在后脑中，主要的PZ显然代表发育中小脑的持续胚胎次级基质层。在前脑中，PZ与嗅球的脑室区和腹侧端脑区（"软腭下"）、背侧端脑区（"软腭"）、视前区、腹侧丘脑、背侧丘脑、上丘脑、顶盖前、后结节和下丘脑相关。间脑PZ根据神经节组织（一个联脑、一个后部和一个前部脑实质神经节：p1、p2和p3）进行分组。因此，次级前脑（端脑和下丘脑）的PZ属于神经节p4 - 6，但不会形成立即识别的序列模式。前脑PZ与Bergquist和Kallen（[1954] J. Comp. Neurol. 100：627 - 659），有丝分裂后神经元从其迁移到其最终目的地的形态发生场。神经学比较杂志436：92 - 110，2001. (C)2001 Wiley-Liss，Inc.
Zones containing actively dividing cells (proliferation zones: PZs), in the brain of adult three-spined sticklebacks, were identified by autoradiographic detection of H-3-thymidine and immunocytochemical detection of the thymidine analogue 5'-bromodeoxyuridine (BrdU), singly or in combination, and by immunocytochemical detection of proliferating cell nuclear antigen (PCNA) by monoclonal antibodies. The PZs are associated with boundaries between adult brain regions, as well as with defined morphofunctional subdivisions. PZs are located at the border between the telencephalon and diencephalon, and at the border between the mesencephalon and the rhombencephalon. In the midbrain, the PZ follows the dorsomedial, caudal, and ventrolateral aspects of each tectal hemisphere, extending over the caudal aspect of the torus semicircularis to the nucleus lateralis valvulae. In the hindbrain, the major PZ apparently represents the persisting embryonic secondary matrix layer of the developing cerebellum. In the forebrain, the PZs are associated with the ventricular zones of the olfactory bulbs and ventral telencephalic area ("subpallium"), dorsal telencephalic area ("pallium"), preoptic region, ventral thalamus, dorsal thalamus, epithalamus, pretectum, posterior tuberculum, and the hypothalamus. The diencephalic PZs are parcellated according to a neuromeric organisation (a synencephalic, a posterior, and an anterior parencephalic neuromere: p1, p2, and p3). The PZs of the secondary prosencephalon (telencephalon and hypothalamus) thus would belong to neuromeres p4-6, but do not form an immediately recognised serial pattern. The prosencephalic PZs correlate well with parts of embryonic migration areas as defined by Bergquist and Kallen ([1954] J. Comp. Neurol. 100:627-659), morphogenetic fields from which postmitotic neurones migrate to their final destination. J. Comp. Neurol. 436:92-110, 2001. (C) 2001 Wiley-Liss, Inc.