Polyethyleneimine-bonded phases in the separation of proteins by capillary electrophoresis.
Polyethyleneimine-bonded phases in the separation of proteins by capillary electrophoresis.
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DOI：
10.1016/s0021-9673(01)90205-5
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发表时间：
1990-09
期刊：
影响因子：
--
通讯作者：
J. Towns;F. Regnier
中科院分区：
文献类型：
--
作者：
J. Towns;F. Regnier
研制了一种亲水性、带正电荷、耐用的毛细管电泳涂料。聚乙烯亚胺被吸附到熔融石英毛细管的内壁上，并且被吸附的涂层交联成稳定的层。由于带正电的涂层引起的电渗流的逆转，聚乙烯亚胺涂层二氧化硅的毛细管产生了独特的分离。所得涂层在pH 2-12范围内是稳定的，并且可以在宽pH范围内使用，而电渗流没有实质性变化。需要高分子量聚合物来提供厚的涂层，以掩盖壁上的硅烷醇基团。使用50 cm长的毛细管快速有效地分离蛋白质，回收率良好。
A hydrophilic, positively charged, durable coating has been developed for cap- illary electrophoresis of macromolecules. Polyethyleneimine is adsorbed to the inner wall of fused silica capillaries and the adsorbed coating cross-linked into a stable layer. Capillaries of polyethyleneimine-coated silica gave unique separations owing to the reversal of electro-osmotic flow caused by the positively charged coating. The resulting coating was stable from pH 2-12 and could be used over a wide pH range without substantial change in electro-osmotic flow. High-molecular-weight polymers were needed to give thick coatings which mask silanol groups on the wall. Proteins were resolved quickly and efficiently with good recovery using capillaries of 50 cm in length.