Chicken intestinal aminopeptidase: Partial sequence of the gene, expression and activity
Chicken intestinal aminopeptidase: Partial sequence of the gene, expression and activity
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DOI：
10.1093/ps/79.1.41
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发表时间：
2000-01-01
期刊：
影响因子：
4.4
通讯作者：
Uni, Z
中科院分区：
文献类型：
--
作者：
Gal-Garber, O;Uni, Z
氨肽酶是膜结合金属肽酶家族的成员，其在肠细胞的刷状缘膜上以高水平表达。由于肉鸡的快速生长依赖于日粮中氨基酸的供应，因此对在蛋白质消化中起核心作用的肠道氨肽酶的研究很重要。本研究首次报道了鸡小肠氨肽酶的部分cDNA的分离和1.7kb cDNA片段的测序。该基因通过逆转录聚合酶链反应分离，使用6个引物从氨肽酶基因的保守区域中选择。从凝胶中提取扩增片段，纯化并测序。通过使用该鸡cDNA作为探针，北方印迹分析显示在鸡十二指肠、空肠和回肠组织中约3.5 kb的转录物。回肠组织中氨肽酶的RNA表达和活性均高于十二指肠和空肠。
Aminopeptidases are members of a membrane-bound metallopeptidase family that are expressed at a high level on the brush-border membrane of enterocytes. Because the rapid growth of meat-type chickens depends on the dietary supply of amino acids, a study of intestinal aminopeptidases, which play a central role in protein digestion, is important. This study is the first reported isolation of the partial cDNA of chicken intestinal aminopeptidase and sequencing of a 1.7-kb cDNA fragment. The gene was isolated by reverse transcriptase polymerase chain reaction using six primers chosen from conserved regions of the aminopeptidase genes. Amplified fragments were extracted from the gel, purified, and sequenced. By using this chicken cDNA as a probe, northern blot analysis revealed a transcript of approximately 3.5 kb in the chicken duodenum, jejunum, and ileum tissues. Higher RNA expression and activity of aminopeptidase were found in the ileum tissue compared with the duodenum and jejunum segments.