Characterization of tissue- and cell-type-specific expression of a novel human septin family gene, Bradeion.
Characterization of tissue- and cell-type-specific expression of a novel human septin family gene, Bradeion.
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DOI：
10.1006/bbrc.2001.5413
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发表时间：
2001-08
影响因子：
3.1
通讯作者：
Manami Tanaka;Tomoo Tanaka;Tomoo Tanaka;H. Kijima;H. Kijima;J. Itoh;T. Matsuda;S. Hori;M. Ya
中科院分区：
文献类型：
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作者：
Manami Tanaka;Tomoo Tanaka;Tomoo Tanaka;H. Kijima;H. Kijima;J. Itoh;T. Matsuda;S. Hori;M. Ya
在改变细胞命运的过程中发生基因子集的表达变化，即，衰老、细胞死亡和致癌作用。这些变化同时提供了良好的候选者作为监测癌症的生物标志物。我们用单克隆抗体CE5从成人脑cDNA文库中鉴定了一个新的人septin家族基因Bradeion。北方印迹和原位杂交分析表明，Bradeion有两种不同的转录本，约2.2和1.7 kb长（分别为α和β），主要在脑中，在心脏中略有表达，在任何胎儿器官中均不表达。单倍型分析将该基因定位于17q23。该基因含有在胞质分裂和细胞分离所必需的septin家族基因中高度保守的GT3基序。β形式的转录物缺乏疏水性区域，这表明这种形式通过独特的RNA剪接由单个Bradeion基因产生。有趣的是，这种脑特异性Bradeion基因也在两种人类癌症中表达，结肠直肠癌和恶性黑色素瘤。在患者的肿瘤样品和体外培养的人癌细胞系中均证实了正常Bradeion α和β转录物的异位表达。因此，Bradeion作为肿瘤特异性和选择性标记物提供了有价值的工具。
Expression changes in subsets of genes occur in the course of altering cell fates, i.e., aging, cell death, and carcinogenesis. These changes simultaneously provide the good candidate as a biomarker for monitoring cancer. We have identified a novel human septin family gene, Bradeion, from adult brain cDNA library by a monoclonal antibody CE5. Northern blot and in situ hybridization analysis showed that Bradeion has two distinct transcripts, approximately 2.2 and 1.7 kb length (alpha and beta, respectively) mainly in brain and slightly in heart, and no expression in any fetal organs. Haplotype analysis placed the gene location at 17q23. The gene contains GTPase motifs highly conserved in the septin family genes that are essential for cytokinesis and cell separation. The transcript of beta form lacks a hydrophobic region, which suggests that this form arises from a single Bradeion gene through unique RNA splicing. Interestingly, this brain-specific Bradeion gene is also expressed in two human cancers, colorectal cancer and malignant melanoma. Ectopic expression of normal Bradeion alpha and beta transcripts were confirmed both in patients' tumor samples and in in vitro cultured human cancer cell lines. Thus the Bradeion provides valuable tools as a tumor-specific and selective marker.