Bead-based multianalyte flow immunoassays: the cytometric bead array system.
Bead-based multianalyte flow immunoassays: the cytometric bead array system.
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DOI：
10.1007/978-1-59745-323-3_9
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Apgar, John
中科院分区：
文献类型：
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作者：
Varro, Rudolf;Chen, Roy;Apgar, John
分析型细胞术具有超越细胞分析的显著潜力。流式细胞仪根据颗粒的内在性质有效区分尺寸均匀的颗粒的固有能力为多重珠检测提供了基础。该技术可用于设计免疫测定、蛋白质印迹样抗体测定和核酸杂交测定。本章重点介绍免疫分析应用。多重微珠检测是近年来发展起来的一个新的、越来越受欢迎的流式细胞术领域，成为酶联免疫吸附测定的一个很好的替代方法，用于有效地评价分析物组。本章提供了有关两种微珠平台（BD Cytometric Bead Array试剂盒和BD Cytometric Bead Array Flex Set Assay）的详细信息。
Analytical cytometry has significant potential beyond cellular analysis. The inherent capability of flow cytometers to efficiently discriminate between uniformly sized particles based on their intrinsic properties provides the foundation for multiplex bead assays. The technology can be exploited in designing immunoassays, Western blot-like antibody assays, and nucleic acid hybridization assays. This chapter focuses on immunoassay applications. The multiplex bead assays have recently evolved as a new and increasingly popular area for flow cytometry, becoming a good alternative to enzyme-linked immunosorbent assay for efficient evaluation of panels of analytes. This chapter provides detailed information about two bead platforms, the BD Cytometric Bead Array kits and the BD Cytometric Bead Array Flex Set Assays.