Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source
Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source
复制标题
DOI：
10.1038/s41598-019-57376-w
复制
发表时间：
2020-01-16
影响因子：
4.6
通讯作者：
Fouras, Andreas
中科院分区：
文献类型：
--
作者：
Murrie, Rhiannon P.;Werdiger, Freda;Fouras, Andreas
肺健康的大多数测量独立地评估整体肺功能或局部肺结构。区域性地测量气流和肺功能的能力将提供更具体和生理上集中的手段，通过该手段来评估和跟踪临床前和临床环境中的肺部疾病。用于实现局部肺功能测量的一种方法是经由相位对比X射线成像（PCXI），其已被证明提供小动物的肺和气道的高灵敏度、高分辨率图像。PCXI提供的详细图像允许应用四维X射线测速仪（4DxV）跟踪肺组织运动并提供局部肺功能的定量信息。然而，直到最近，还需要同步加速器设施来产生高度相干、高通量的X射线，这是以足够高的帧速率实现肺PCXI以捕获肺运动所需的。本文介绍了第一个翻译的4DxV技术从同步加速器设施到实验室设置通过使用液态金属射流微焦点X射线源。该源可以提供PCXI所需的相干性和足够的X射线通量，以在呼吸周期期间对肺组织运动的动态进行成像，从而能够生成与4DxV分析兼容的图像。我们展示了使用这种技术可以在活体小鼠体内捕获的测量结果，包括区域气流和组织扩张。这些测量可以为小动物的生理和生物医学研究提供信息，并有助于开发新的呼吸治疗方法。
Most measures of lung health independently characterise either global lung function or regional lung structure. The ability to measure airflow and lung function regionally would provide a more specific and physiologically focused means by which to assess and track lung disease in both pre-clinical and clinical settings. One approach for achieving regional lung function measurement is via phase contrast X-ray imaging (PCXI), which has been shown to provide highly sensitive, high-resolution images of the lungs and airways in small animals. The detailed images provided by PCXI allow the application of four-dimensional X-ray velocimetry (4DxV) to track lung tissue motion and provide quantitative information on regional lung function. However, until recently synchrotron facilities were required to produce the highly coherent, high-flux X-rays that are required to achieve lung PCXI at a high enough frame rate to capture lung motion. This paper presents the first translation of 4DxV technology from a synchrotron facility into a laboratory setting by using a liquid-metal jet microfocus X-ray source. This source can provide the coherence required for PCXI and enough X-ray flux to image the dynamics of lung tissue motion during the respiratory cycle, which enables production of images compatible with 4DxV analysis. We demonstrate the measurements that can be captured in vivo in live mice using this technique, including regional airflow and tissue expansion. These measurements can inform physiological and biomedical research studies in small animals and assist in the development of new respiratory treatments.