Quantitative microassay in cell culture for enterotoxin of Shigella dysenteriae.
Quantitative microassay in cell culture for enterotoxin of Shigella dysenteriae.
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细胞培养中痢疾志贺氏菌肠毒素的定量微量测定。
DOI：
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发表时间：
1972
影响因子：
6.4
通讯作者：
S. Hirschman
中科院分区：
文献类型：
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作者：
G. Keusch;M. Jacewicz;S. Hirschman
志贺氏菌肠毒素对HeLa细胞单层具有细胞毒性，并导致细胞从玻璃表面快速脱离。过夜孵育期间分离的细胞数量与培养基中存在的毒素量直接相关。基于这些观察结果，定量微量测定，可重复检测的毒素的纳克数量，开发。该技术包括计数暴露于毒素后仍固定在玻璃上的细胞数量，也可适用于其他细胞毒性细菌产物，如白喉棒状杆菌的外毒素。
Shigella dysenteriae enterotoxin is cytotoxic to HeLa-cell monolayers and results in rapid detachment of cells from glass surfaces. The number of cells that detach during incubation overnight is directly related to the quantity of toxin present in the medium. Based on these observations, a quantitative microassay, which reproducibly detected subnanogram quantities of toxin, was developed. The technique involves enumeration of the number of cells that remain fixed to glass after exposure to toxin and may also be adaptable to other cytotoxic bacterial products, such as the exotoxin of Corynebacterium diphtheriae.