Charge matters.
Charge matters.
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DOI：
10.1007/s12551-016-0229-3
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发表时间：
2016-12-01
影响因子：
--
通讯作者：
Laue, Thomas
中科院分区：
文献类型：
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作者：
Laue, Thomas
我第一次读到唐·温佐的论文是在20世纪70年代初，当时我还是加里阿克和大卫·伊潘提斯实验室的研究生。唐对开发表征蛋白质-蛋白质相互作用的方法的兴趣与这些实验室的兴趣相匹配，特别是在色谱和分析超离心方面。多年来，我有机会在各种会议上见到唐，主要是在澳大利亚，并讨论了热力学非理想性的来源和测量。20世纪90年代的一个下午，当我抬头看到唐站在我在新罕布什尔州大学（UNH）办公室的门口时，想象一下我的惊讶吧。原来，他的一个女儿正在UNH攻读化学博士学位，唐正在拜访她，作为环球旅行的一部分。我们匆忙安排了一个研讨会，请他过来吃晚饭。在接下来的几年里，唐偶尔会去看他的女儿，总是顺便到我的办公室来聊聊他的最新作品。他从来没有宣布过他的来访，就好像他住在隔壁的镇上，只是想停下来打个招呼。我们的谈话集中在蛋白质-蛋白质相互作用以及非理想性在控制它们中的作用。
I first read Don Winzor’s papers in the early 1970s while a graduate student in Gary Acker’s and David Yphantis’s labs. Don’s interest in developing methods for characterizing protein–protein interactions matched those labs’ interests, particularly with respect to chromatography and analytical ultracentrifugation. Over the years, I had the opportunity to meet Don at various conferences, mostly in Australia, and to discuss the sources and measurement of thermodynamic nonideality. Imagine my surprise, then, when one afternoon in the 1990s, I looked up to find Don standing in the doorway of my office at the University of New Hampshire (UNH). It turned out that one of his daughters was working on her chemistry PhD at UNH, and Don was paying her a visit as part of an around-theworld tour. A seminar was hastily arranged, and we had him over for supper. Over the next few years, Don would occasionally visit his daughter, always dropping by my office to chat about his latest work. He never announced his visits—it was as though he lived in the next town and just wanted to stop in to say hello. Our chats focused on protein–protein interactions and the role of nonideality in governing them.