The zebrafish Annexin gene family
The zebrafish Annexin gene family
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DOI：
10.1101/gr.479603
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发表时间：
2003-06-01
期刊：
影响因子：
7
通讯作者：
Halpern, ME
中科院分区：
文献类型：
--
作者：
Farber, SA;De Rose, RA;Halpern, ME
膜联蛋白(Annexins，ANX)是一个钙和磷脂结合蛋白家族，参与许多细胞过程，包括通道形成、膜融合、囊泡运输和磷脂酶A活性的调节。作为了解活体功能的第一步，我们克隆了11个斑马鱼ANX基因。通过对斑马鱼基因文库的筛选，获得了4个斑马鱼基因(Xan1a、Xax2a、Xang5和Xang11a)。对于这些基因，使用全长cDNA序列对斑马鱼基因组资源计划产生的212个EST序列进行聚类。EST分析揭示了随后被克隆的另外七个anx基因。利用斑马鱼辐射杂交仪表板确定了所有11个基因的遗传图谱位置。斑马鱼和人类基因的序列和同线关系表明，这11个基因代表了人类anx1，2，4，5，6，11，13的同源基因，这表明几个斑马鱼anx基因是斑马鱼和哺乳动物基因组分化后产生的重复基因。斑马鱼anx基因在胚胎和幼体阶段的广泛组织中表达。对重复基因的表达模式的分析表明，既有冗余又有分歧，最相似的基因具有几乎相同的组织特异性表达模式，而不太相似的重复基因没有重叠。最近复制的anx基因的基因表达差异可以解释为什么高度相关的并列基因保持在基因组中，而不是迅速成为假基因。
The Annexins (ANXs) are a family of calcium- and phospholipid-binding proteins that have been implicated in many cellular processes, including channel formation, membrane fusion, vesicle transport, and regulation of phospholipase A, activity. As a first step toward understanding in Vivo function, we have cloned 11 zebrafish anx genes. Four genes (anx1a, anx2a, anx5, and anx11a) were identified by screening a zebrafish cDNA library with a Xenopus anx2 fragment. For these genes, full-length cDNA sequences were used to cluster 212 EST sequences generated by the Zebrafish Genome Resources Project. The EST analysis revealed seven additional anx genes that were subsequently cloned. The genetic map positions of all 11 genes were determined by using a zebrafish radiation hybrid panel. Sequence and syntenic relationships between zebrafish and human genes indicate that the 11 genes represent orthologs of human anx1,2,4,5,6,11,13, and suggest that several zebrafish anx genes resulted from duplications that arose after divergence of the zebrafish and mammalian genomes. Zebrafish anx genes are expressed in a wide range of tissues during embryonic and larval stages. Analysis of the expression patterns of duplicated genes revealed both redundancy and divergence, with the most similar genes having almost identical tissue-specific patterns of expression and with less similar duplicates showing no overlap. The differences in gene expression of recently duplicated anx genes could explain why highly related paralogs were maintained in the genome and did not rapidly become pseudogenes.