Synthesis of Cell Wall Microfibrils in vitro by a "Soluble" Chitin Synthetase from Mucor rouxii
Synthesis of Cell Wall Microfibrils in vitro by a "Soluble" Chitin Synthetase from Mucor rouxii
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鲁氏毛霉“可溶性”几丁质合成酶体外合成细胞壁微纤维
DOI：
10.1126/science.186.4161.357
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发表时间：
1974
期刊：
影响因子：
56.9
通讯作者：
Salomon Bartnicki
中科院分区：
文献类型：
--
作者：
Jos é Ruiz;Salomon Bartnicki
通过接触酶底物(尿苷二磷酸N-乙酰-D-氨基葡萄糖)和激活剂(N-乙酰-D-氨基葡萄糖)，从富膜组份中分离出一种“可溶”形式的甲壳素合成酶。溶解的酶催化甲壳素微纤维的合成，即使不是完全相同，也与真菌在体内形成的甲壳素微纤维相似。因此，在没有活细胞或细胞膜的情况下，细胞壁微纤维大量形成。
A "soluble" form of chitin synthetase was separated from a membrane-rich fraction by exposure to the enzyme substrate (uridine diphosphate N-acetyl-D-glucosamine) and activator (N-acetyl- D-glucosamine). The solubilized enzyme catalyzed the synthesis of chitin microfibrils similar, if not identical, to those formed in vivo by the fungus. Cell wall microfibrils were thus abundantly formed in the absence of a living cell or its membranes.