SIMPLE MENDELIAN INHERITANCE OF REITERATED RIBOSOMAL DNA OF YEAST
SIMPLE MENDELIAN INHERITANCE OF REITERATED RIBOSOMAL DNA OF YEAST
复制标题
DOI：
10.1073/pnas.74.11.5091
复制
发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
BOTSTEIN, D
中科院分区：
文献类型：
--
作者：
PETES, TD;BOTSTEIN, D
一株二倍体酵母（Saccharomyces cerevisiae）具有2种高度重复的核糖体DNA杂合。这些形式可以通过位点特异性限制性内切酶EcoRI消化后产生的片段模式来区分。通过四分体分析确定核糖体DNA的遗传方式。在分析的14个四分体中，12个清楚地显示核糖体DNA形式作为一个单一的孟德尔单位分离。对这一结果最简单的解释是，酵母细胞的大约100个核糖体DNA基因拷贝全部位于1条染色体上，这些基因内的减数分裂重组受到抑制。14个四分体中有两个显示出预期的核糖体DNA内有丝分裂重组的分离模式。[DNA探针是由大肠杆菌菌株制备的]。
A diploid strain of yeast (Saccharomyces cerevisiae) was heterozygous for 2 forms of the highly repetitious ribosomal DNA. These forms could be distinguished by the pattern of fragments produced after digestion with the site-specific restriction endonuclease EcoRI. The mode of inheritance of ribosomal DNA was determined by tetrad analysis. Of 14 tetrads analyzed, 12 clearly showed the ribosomal DNA forms segregating as a single Mendelian unit. The simplest interpretation of this result is that all of the approximately 100 copies of the ribosomal DNA genes of the yeast cell are located on 1 chromosome and that meiotic recombination within these genes is suppressed. Two of the 14 tetrads showed the segregation patterns expected as the result of mitotic recombination within the ribosomal DNA. [The DNA probe was prepared from an Escherichia coli strain].