Vaginal CD4+ T cells express high levels of CCR5 and are rapidly depleted in simian immunodeficiency virus infection
Vaginal CD4+ T cells express high levels of CCR5 and are rapidly depleted in simian immunodeficiency virus infection
复制标题
DOI：
10.1086/368386
复制
发表时间：
2003-03-01
影响因子：
6.4
通讯作者：
Lackner, AA
中科院分区：
文献类型：
--
作者：
Veazey, RS;Marx, PA;Lackner, AA
在世界范围内，大多数人类免疫缺陷病毒-1病例通过异性传播发生，但对阴道粘膜中CD 4（+）T细胞的表型知之甚少。在本研究中，淋巴细胞进行了比较，从淋巴结，血液，阴道从未感染和猴免疫缺陷病毒（SIV）感染的猕猴。在成熟的雌性猕猴中，54%-67%的阴道CD 4（+）T细胞表达C趋化因子受体5（CCR 5），而84%-99%共表达CXC趋化因子受体4。相反，只有4.4%-14.8%的外周血和2.4%-13%的淋巴结CD 4（+）T细胞共表达CCR 5。此外，CCR 5平均通道荧光显着较高的CD 4细胞从阴道，与血液相比。在静脉感染SIV的猕猴中，在阴道中观察到CD 4（+）T细胞的快速耗竭，特别是在CCR 5（+）CD 4（+）亚群中。这表明大量表达高水平CCR 5的CD 4（+）T细胞存在于阴道内，并且这些细胞优先被SIV感染清除。
Worldwide, the majority of human immunodeficiency virus-1 cases occur through heterosexual transmission, yet little is known regarding the phenotype of CD4(+) T cells in the vaginal mucosa. In the present study, lymphocytes were compared from the lymph nodes, blood, and vagina from uninfected and simian immunodeficiency virus (SIV)-infected macaques. In mature female macaques, 54%-67% of the vaginal CD4(+) T cells expressed C chemokine receptor 5 (CCR5), whereas 84%-99% coexpressed CXC chemokine receptor 4. In contrast, only 4.4%-14.8% of peripheral blood and 2.4%-13% of lymph-node CD4(+) T cells coexpressed CCR5. Moreover, CCR5 mean channel fluorescence was significantly higher on CD4 cells from the vagina, compared with those from blood. In macaques intravenously infected with SIV, rapid depletion of CD4(+) T cells was observed in the vagina, particularly among the CCR5(+) CD4(+) subset. This demonstrates that large numbers of CD4(+) T cells expressing high levels of CCR5 reside within the vagina and that these cells are preferentially targeted for elimination by SIV infection.