Inheritance and expression of NAD(P)H nitrate reductase in barley
Inheritance and expression of NAD(P)H nitrate reductase in barley
复制标题
大麦NAD(P)H硝酸还原酶的遗传和表达
DOI：
10.1007/bf00247547
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发表时间：
1987
影响因子：
5.4
通讯作者：
A. Kleinhofs
中科院分区：
文献类型：
--
作者：
R. L. Warner;K. R. Narayanan;A. Kleinhofs
大麦中存在NADH特异酶和NAD(P)H双特异硝酸还原酶。野生型叶片只有NADH专一性的酶，而NADH硝酸还原酶结构基因(Nar1)缺陷的突变体具有NAD(P)H双特异酶。从一个NADH硝酸还原酶结构基因缺失的品系(Nar1a)中分离到1个NAD(P)H硝酸还原酶缺陷突变株。双突变体(nar1a；nar7w)缺乏NAD(P)H硝酸还原酶活性，黄嘌呤脱氢酶和亚硝酸还原酶活性与tanar1a相似。该突变体的NAD(P)H硝酸还原酶活性受一个共显性基因nar7控制。Thenar7基因座似乎是NAD(P)H硝酸还原酶结构基因，与Thenar1没有紧密连锁。通过对野生型与nar1a；nar7w杂交后代的分离，获得了一个在根和叶中都具有与野生型相同的NADH硝酸还原酶活性但在根中缺乏NADPH硝酸还原酶活性的品系。假定此行具有genotypeNar1Nar1nar7nar7。NADH和NAD(P)H硝酸还原酶抗血清对NADH和NAD(P)H硝酸还原酶的不同失活表明，野生型幼苗的根同时具有硝酸还原酶。因此，NAN7控制大麦根和叶中的NAD(P)H硝酸还原酶。
NADH-specific and NAD(P)H bispecific nitrate reductases are present in barley (Hordeum vulgareL.). Wild-type leaves have only the NADH-specific enzyme while mutants with defects in the NADH nitrate reductase structural gene (nar1) have the NAD(P)H bispecific enzyme. A mutant deficient in the NAD(P)H nitrate reductase was isolated in a line (nar1a) deficient in the NADH nitrate reductase structural gene. The double mutant (nar1a;nar7w) lacks NAD(P)H nitrate reductase activity and has xanthine dehydrogenase and nitrite reductase activities similar tonar1a. NAD(P)H nitrate reductase activity in this mutant is controlled by a single codominant gene designatednar7. Thenar7 locus appears to be the NAD(P)H nitrate reductase structural gene and is not closely linked tonar1. From segregating progeny of a cross between the wild type andnar1a;nar7w, a line was obtained which has the same NADH nitrate reductase activity as the wild type in both the roots and leaves but lacks NADPH nitrate reductase activity in the roots. This line is assumed to have the genotypeNar1Nar1nar7nar7. Roots of wild type seedlings have both nitrate reductases as shown by differential inactivation of the NADH and NAD(P)H nitrate reductases by a monospecific NADH-nitrate reductase antiserum. Thus,nar7 controls the NAD(P)H nitrate reductase in roots and in leaves of barley.