Eosinophils cocultured with endothelial cells have increased survival and functional properties.
Eosinophils cocultured with endothelial cells have increased survival and functional properties.
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与内皮细胞共培养的嗜酸性粒细胞具有增加的存活率和功能特性。
DOI：
10.1126/science.3110954
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Stevens,RL
中科院分区：
文献类型：
--
作者：
Rothenberg,ME;OwenJr,WF;Silberstein,DS;Soberman,RJ;Austen,KF;Stevens,RL
人外周血嗜酸性粒细胞，细胞往往与过敏性和寄生虫病，维持在体外至少14天，当他们与牛内皮细胞共培养，并为至少7天，当与牛或人内皮细胞衍生的条件培养基培养。共培养的嗜酸性粒细胞变得低密度，并产生约3倍的白三烯C4激活后与钙离子载体和杀死约3倍的抗体包被的幼虫ofSchistosoma mansonias新鲜分离的normodense嗜酸性粒细胞。这些细胞可以通过与内皮细胞共培养在体外维持，并且令人惊讶的发现是，共培养的嗜酸性粒细胞具有与过敏性和其他疾病患者中的嗜酸性粒细胞相似的生物化学、细胞毒性和密度特性，这将有助于研究这些细胞在健康和疾病中的调节和作用。
Human peripheral blood eosinophils, cells often associated with allergic and parasitic diseases, were maintained in vitro for at least 14 days when they were cocultured with bovine endothelial cells and for at least 7 days when cultured with either bovine or human endothelial cell-derived conditioned medium. The cocultured eosinophils became hypodense and generated about three times as much leukotriene C4upon activation with calcium ionophore and killed about three times as many antibody-coated larvae ofSchistosoma mansonias freshly isolated normodense eosinophils. That these cells can be maintained in vitro by coculture with endothelial cells, and the surprising finding that the cocultured eosinophils have biochemical, cytotoxic, and density properties similar to those of eosinophils in patients with allergic and other disorders, will facilitate investigation of the regulation and role of these cells in health and disease.