Toroidal coil counter-current chromatography study of the mass transfer rate of proteins in aqueous-aqueous polymer phase system
Toroidal coil counter-current chromatography study of the mass transfer rate of proteins in aqueous-aqueous polymer phase system
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DOI：
10.1016/s0021-9673(97)01171-0
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发表时间：
1998-04-10
影响因子：
4.1
通讯作者：
Qi, L
中科院分区：
文献类型：
--
作者：
Ito, Y;Matsuda, K;Qi, L
研究了蛋白质样品在逆流色谱(CCC)聚合物相分配过程中谱带过度展宽的原因。用简易旋转装置测定了重铬酸钾、亚甲基蓝、溶菌酶、卵白蛋白和人血清白蛋白等5种样品的传质速率。结果表明，这些样品的传质速率与其分子质量密切相关，分子质量越大，传质速率越低。这些发现也与CCC在同一溶剂体系中对这些样品的分配效率一致。基于蛋白质分子通过界面的传质阻力，可以合理地解释Coriolis力在环形螺旋离心机分离蛋白质中的有利作用：我们推测，当Coriolis力平行于有效线圈段作用时，它可以使流动相分散到固定相中，从而产生较大的界面面积，从而加快蛋白质样品的传质速度。(C)1998年爱思唯尔科学公司。
The cause of excessive band broadening of protein samples in polymer phase partitioning by counter-current chromatography (CCC) was investigated. A simple rotary device was constructed to measure the mass transfer rates of five samples including potassium dichromate, methylene blue, lysozyme, ovalbumin and human serum albumin. The results indicated that the mass transfer rates of these samples are closely correlated with their molecular masses: the higher the molecular mass, the lower the mass transfer rate. These findings are also consistent with the partition efficiencies of these samples in the same solvent system by CCC. The beneficial effect of the Coriolis force demonstrated in protein separations by the toroidal coil centrifuge may be reasonably explained on the basis of the mass transfer resistance of protein molecules through the interface: we speculate that when the Coriolis force acts parallel to the effective coil segment it can produce large interfacial areas by dispersing the mobile phase into the stationary phase, thus accelerating the mass transfer rate of protein samples. (C) 1998 Elsevier Science B.V.