MiR-216a and miR-216b as markers for acute phased pancreatic injury.
MiR-216a and miR-216b as markers for acute phased pancreatic injury.
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DOI：
10.2220/biomedres.34.179
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发表时间：
2013-08
期刊：
影响因子：
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通讯作者：
Kosuke Endo;Huachun Weng;Naoko Kito;Yasue Fukushima;N. Iwai
中科院分区：
文献类型：
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作者：
Kosuke Endo;Huachun Weng;Naoko Kito;Yasue Fukushima;N. Iwai
microRNA（miRNAs）是一种内源性小分子RNA（长度为18-ss 23个核苷酸），可调控基因的表达。最近，血浆miRNA已被研究作为各种疾病的生物标志物。在本研究中，我们探索了细胞或组织特异性miRNA，并评估了miRNA谱用于鉴定组织损伤生物标志物的适用性。对各种人类和大鼠组织的miRNA分析发现了几种可能具有组织特异性表达的候选miRNA，其中一些已经有报道。在本研究中，我们专注于胰腺特异性miRNAs，miR-216 a和miR-216 b。激光显微切割显示，与胰岛相比，miR-216 a和216 b主要在胰腺的腺泡细胞中表达。在L-精氨酸诱导的急性胰腺炎大鼠模型中，miR-216 a和miR-216 b的血浆浓度显著增加。目前的结果已经证实，在各种细胞中的miRNA表达谱可用于提供细胞或组织特异性损伤的生物标志物。
MicroRNAs (miRNAs) are endogenous small RNAs (length, 18-ss23 nucleotides) that regulate gene expression. Recently, plasma miRNAs have been investigated as biomarkers for various diseases. In the present study, we explored cell- or tissue-specific miRNAs and assessed the applicability of miRNA profiling for identifying biomarkers of tissue injuries. miRNA analyses in various human and rat tissues identified several candidate miRNAs with possible tissue-specific expression, some of which have already been reported. In the present study, we focused on pancreas-specific miRNAs, miR-216a and miR-216b. Laser microdissection revealed that miR-216a and 216b were predominantly expressed in acinar cells of the pancreas as compared to Langerhans' islet. Plasma concentrations of miR-216a and miR-216b considerably increased in a rat model of L-arginineinduced acute pancreatitis. The current results have confirmed that miRNA expression profiling in various cells is useful for providing biomarkers for cell- or tissue-specific injuries.