Preparation of water-soluble antimicrobial zein nanoparticles by a modified antisolvent approach and their characterization
Preparation of water-soluble antimicrobial zein nanoparticles by a modified antisolvent approach and their characterization
复制标题
改进的反溶剂方法制备水溶性抗菌玉米醇溶蛋白纳米颗粒及其表征
DOI：
10.1016/j.jfoodeng.2013.05.038
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发表时间：
2013-11-01
影响因子：
5.5
通讯作者：
Wen, Shao-Hong
中科院分区：
文献类型：
--
作者：
Li, Kang-Kang;Yin, Shou-Wei;Wen, Shao-Hong
我们开发了一种简单的反溶剂方法，通过将酪蛋白酸钠（SC）直接倒入玉米蛋白溶液中来制备玉米蛋白/SC纳米颗粒。纳米颗粒呈球形，冷冻干燥后在去离子水中具有再分散性。纳米粒子对百里香酚具有很强的负载能力，且在8个月以上的储存时间内保持稳定。研究了百里香负载纳米颗粒的形态特征、抗菌和抗氧化性能以及释放谱。在琼脂扩散实验中，百里香酚负载使纳米颗粒对被测细菌的抗菌活性以及DPPH自由基清除活性呈剂量依赖性。这些纳米颗粒在37℃的新鲜M-H肉汤中也能有效延缓金黄色葡萄球菌的生长。百里香酚的添加导致纳米颗粒的大小略有增加，并且不影响纳米颗粒的再分散性。纳米颗粒对百里香酚的释放具有缓释作用，百里香酚的释放动力学可描述为两步双相过程。(C) 2013 Elsevier Ltd.版权所有。
We developed a simple antisolvent procedure to prepare core/shell zein/SC nanoparticles by directly pouring sodium caseinate (SC) into zein solutions. The nanoparticles are spherical in shape, possessing the re-dispersibility in de-ionized water after freeze-drying. The nanoparticles possessed strong loading capacity for thymol, and were stable over 8 months storage. Morphological attributes, antimicrobial and antioxidant properties as well as release profiles of thymol-loaded nanoparticles were investigated. Thymol loadings gave the nanoparticles antimicrobial activity against tested bacteria on agar diffusion assay as well as DPPH radical scavenging activity, in a dose-dependent manner. These nanoparticles were also effective in delaying the growth of Staphylococcus aureus in fresh M-H broth at 37 degrees C. Thymol loading resulted in a slight increase in particle size, and did not affect the re-dispersibility of the nanoparticles. The nanoparticle sustained the release of thymol, and the release kinetic of thymol can be described as a two-step biphasic process. (C) 2013 Elsevier Ltd. All rights reserved.