Characterization of propiconazole field-resistant isolates of Ustilaginoidea virens
Characterization of propiconazole field-resistant isolates of Ustilaginoidea virens
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丙环唑和绿黑粉菌抗田分离株的鉴定
DOI：
10.1016/j.pestbp.2018.11.013
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发表时间：
--
影响因子：
4.7
通讯作者：
Yongfeng Liu
中科院分区：
文献类型：
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作者：
Yuxin Zhou;Junjie Yu;Xiayan Pan;Mina Yu;Yan Du;Zhongqiang Qi;Rongsheng Zhang;Tianqiang Song;Xiaole Yin;Yongfeng Liu
稻曲病是由稻曲病菌（Ustilaginoidea virens（Cooke）Takah）引起的一种重要病害，对水稻生产造成了严重的质量和数量损失。丙环唑是一种三唑类杀菌剂，属于脱甲基化抑制剂（DMIs）。在中国，它被用来控制RFS。我们以前筛选了158株U。对2015年在江苏省田间采集到的10株绿僵菌进行了鉴定，发现其中2株（分别命名为82和88）对丙环唑具有高度抗性。本研究对6个田间敏感菌株和2个田间抗性菌株的菌丝生长和细胞壁完整性等生理生化特性进行了分析。结果表明，不同的DMIs杀菌剂之间存在交互抗性，而DMIs与QoIs杀菌剂之间不存在交互抗性。我们还分析了适合性，发现88个菌株的致病性比现场敏感菌株强，但82个菌株完全丧失了致病性。CYP51和CYP51编码区上游1000 bp的序列分析显示，与场敏感菌株相比，82中没有突变，但在场抗性分离株88中，在编码区上游154 bp处鉴定出另外两个碱基CC艾德。此外，CYP51基因在所有供试菌株中的表达均显著高于丙环唑。induced.by但82和88的表达量均明显高于田间敏感菌株。我们还发现丙环唑可以抑制田间敏感菌株的麦角甾醇生物合成，但对田间抗性菌株82和88都有刺激作用。鉴于美国的高水平。对丙环唑产生了抗性，田间抗性菌株88的适应性较好，U.必须在稻田监测和管理对DMIs的病毒。
The plant-pathogenic fungus Ustilaginoidea virens (Cooke) Takah causes rice false smut (RFS), which is re-.sponsible for significant quantitative and qualitative losses in rice industry. Propiconazole is a triazole fungicide.which belongs to Demethylation inhibitors (DMIs). It is used to control RFS in China. We previously screened.158 isolates of U. virens collected in the fields in 2015 in Jiangsu province of China, and found two of them were.highly resistant to propiconazole (named 82 and 88, respectively). In this study, we have analyzed the phy-.siological and biochemical characters of six field-sensitive isolates and the two field-resistant isolates, including.mycelial growth and cell wall integrity. We found there was cross-resistance between different DMIs fungicides,.but was no cross-resistance between DMIs and QoIs fungicides. We also analyzed the fitness, and found the.pathogenicity in 88 was stronger than the field-sensitive isolates, but was completely lost in 82. Sequence.analyses of CYP51 and the 1000-bp upstream of CYP51 coding region showed no mutation in 82 compared to the.field-sensitive strains, but two more bases CC were identified at 154-bp upstream of the coding region in the.field-resistant isolate 88. Moreover, the expression of CYP51 gene in all tested isolates was significantly induced.by propiconazole. However, the up-regulation expression level in both 82 and 88 was much higher than that in.the field-sensitive isolates. We also found propiconazole could inhibit the ergosterol biosynthesis in the field-.sensitive isolates, but stimulated it in both field-resistant isolates 82 and 88. Given the high level of U. virens.developing propiconazole resistance and the good fitness of the field-resistant isolate 88, the resistance of U..virens to DMIs must be monitored and managed in rice fields.