Mangrove Sediment Microbiome: Adaptive Microbial Assemblages and Their Routed Biogeochemical Processes in Yunxiao Mangrove National Nature Reserve, China
Mangrove Sediment Microbiome: Adaptive Microbial Assemblages and Their Routed Biogeochemical Processes in Yunxiao Mangrove National Nature Reserve, China
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DOI：
10.1007/s00248-018-1261-6
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发表时间：
2019-07-01
影响因子：
3.6
通讯作者：
Tian, Yun
中科院分区：
文献类型：
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作者：
Lin, Xiaolan;Hetharua, Buce;Tian, Yun
微生物在红树林生态系统中起重要作用。但是，我们对红树林微生物组对高生产率和红树林生态系统中元素有效循环的生态含义一无所知。在这里，我们专注于位于中国东南部云Xiao国家红树林保护区的红树林沉积物，使用16S rRNA基因和shot弹枪元素元素测序方法揭开了红树林微生物组。物理化学分析的特征是Yunxiao红树林沉积物为碳（C） - Rich，Sulfur（S）-Rich和Nitrogen（N）限制环境。然后，系统发育分析对具有意外的高频氯曲线和硝基螺旋体进行了独特的微生物组，似乎是富含S的栖息地中微生物结构的适应性特征。元素组测序分析表明，通过氨化和异化的硝酸盐将N和S循环的代谢途径通过氨化和异化的硝酸盐降低到铵中，以在此N限制的生境中n保护，以及在硫酸盐中脱落的硫酸盐减少以及生成生物硫化物的形成，以生成生物硫化物。避免红树林沉积物中硫化物的生物毒性的资源。此外，甲烷代谢充当将C骑自行车连接到N和S循环的桥梁。进一步鉴定可能的生物地球化学连接器表明综合杆菌，硫磺，硝基螺旋体和Anaerolinea可能会驱动C，N和S循环的耦合。这些结果强调了自适应路由代谢流，这是先前未描述的红树林沉积物微生物组的特性，似乎是该栖息地的定义特征，可能会大大促进红树林生态系统的高生产率，这可以用作健康和生物多样性的指标。红树林生态系统。
Microorganisms play important roles in mangrove ecosystems. However, we know little about the ecological implications of mangrove microbiomes for high productivity and the efficient circulation of elements in mangrove ecosystems. Here, we focused on mangrove sediments located at the Yunxiao National Mangrove Reserve in southeast China, uncovering the mangrove microbiome using the 16S rRNA gene and shotgun metagenome sequencing approaches. Physicochemical assays characterized the Yunxiao mangrove sediments as carbon (C)-rich, sulfur (S)-rich, and nitrogen (N)-limited environment. Then phylogenetic analysis profiling a distinctive microbiome with an unexpected high frequency of Chloroflexi and Nitrospirae appeared to be an adaptive characteristic of microbial structure in S-rich habitat. Metagenome sequencing analysis revealed that the metabolic pathways of N and S cycling at the community-level were routed through ammonification and dissimilatory nitrate reduction to ammonium for N conservation in this N-limited habitat, and dissimilatory sulfate reduction along with polysulfide formation for generating bioavailable S resource avoiding the biotoxicity of sulfide in mangrove sediments. In addition, methane metabolism acted as a bridge to connect C cycling to N and S cycling. Further identification of possible biogeochemical linkers suggested Syntrophobacter, Sulfurovum, Nitrospira, and Anaerolinea potentially drive the coupling of C, N, and S cycling. These results highlighting the adaptive routed metabolism flow, a previously undescribed property of mangrove sediment microbiome, appears to be a defining characteristic of this habitat and may significantly contribute to the high productivity of mangrove ecosystems, which could be used as indicators for the health and biodiversity of mangrove ecosystems.