PRODUCTION AND CHARACTERIZATION OF ARBOVIRUS ANTIBODY IN MOUSE ASCITIC FLUID
PRODUCTION AND CHARACTERIZATION OF ARBOVIRUS ANTIBODY IN MOUSE ASCITIC FLUID
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DOI：
10.4269/ajtmh.1967.16.339
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发表时间：
1967-01-01
影响因子：
3.3
通讯作者：
HETRICK, FM
中科院分区：
文献类型：
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作者：
BRANDT, WE;BUESCHER, EL;HETRICK, FM
从小鼠腹水中获得高效价的虫媒病毒CF抗体。由多次紧密间隔注射组成的初次和二次免疫接种产生的抗体应答与一次初次和一次二次注射后发现的抗体应答相似，但产生了更高滴度但特异性相似的试剂。对于初次免疫，皮下接种比腹膜内接种更有效，而对于二次免疫，则相反。高免疫腹水中CF和HI抗体仅见于7S γ G免疫球蛋白区。在未加热的腹水的γ球蛋白区域的19 S部分中发现AC活性;当从7S γ G-免疫球蛋白中分离时，它是热不稳定的，并且在其存在下是热稳定的。HI试验中的非特异性抑制剂显示为与19 S或7S球蛋白无关的脂蛋白。
Specific high-titered arbovirus CF antibody was obtained in mouse ascitic fluid. Primary and secondary immunization consisting of multiple closely spaced injections produced an antibody response similar to that found after one primary and one secondary injection, but resulted in higher-titered yet similarly specific reagents. Subcutaneous inoculation was shown to be more effective than intraperitoneal inoculation for primary immunization, whereas the reverse was true for secondary immunization. CF and HI antibody in hyperimmune ascitic fluid was found only in the 7S gamma G-immunoglobulin region. AC activity was found in the 19S portion of the gamma globulin region of unheated ascitic fluid; it was heat labile when isolated from 7S gamma G-immunoglobulins and heat stable in their presence. Non-specific inhibitors in the HI test were shown to be lipoproteins that were not associated with 19S or 7S globulins.