Molecular Basis for Resistance to Fenoxaprop in Shortawn Foxtail (Alopecurus aequalis) from China
Molecular Basis for Resistance to Fenoxaprop in Shortawn Foxtail (Alopecurus aequalis) from China
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DOI：
10.1614/ws-d-14-00105.1
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发表时间：
2015-04-01
期刊：
影响因子：
2.5
通讯作者：
Wang, Jinxin
中科院分区：
文献类型：
--
作者：
Guo, Wenlei;Liu, Weitang;Wang, Jinxin
短尾草是一种竞争性的一年生杂草，广泛分布于中国东部、中南部、西南部和黄河流域部分地区。在江苏省江堰市发现1个对非诺沙丙产生抗性的短尾狐种群（JSQT-1）。全株实验确定，抗性种群对非诺沙普罗（93倍）、氯地那福（21倍）、乙氧基啶（107倍）、中硫隆（41倍）和pyroxsulam（12倍）具有高水平抗性；对haloxyfop（8倍）、clethodim（9倍）和pinoxaden（8倍）有中等水平的抗性，对异丙隆无抗性。分子分析证实在耐药群体中存在il -1781- leu突变。使用dCAPS标记检测Ile-1781-Leu突变。97株抗性植株均为1781位点的纯合突变体。本研究首次建立了短尾狐尾对非诺沙丙的抗性，确定了短尾狐尾对其他除草剂的交叉抗性，并阐明了短尾狐尾对非诺沙丙产生抗性的分子基础，至少部分原因是短尾狐尾在其质体ACCase中氨基酸1781位发生了Ile - Leu突变。
Shortawn foxtail is a competitive annual grass weed widely spread in east, south-central, and southwest China and parts of the Yellow River basin. One shortawn foxtail population (JSQT-1) resistant to fenoxaprop was identified in Jiangyan, Jiangsu province. Whole-plant experiments determined that the resistant population conferred high-level resistance to fenoxaprop (93-fold), clodinafop (21-fold), sethoxydim (107-fold), mesosulfuron (41-fold), and pyroxsulam (12-fold); moderate-level resistance to haloxyfop (8-fold), clethodim (9-fold), and pinoxaden (8-fold), and no resistance to isoproturon. Molecular analyses confirmed that the Ile-1781-Leu mutation was present in the resistant population. A dCAPS marker was used to detect the Ile-1781-Leu mutation. All 97 plants of the resistant population analyzed were homozygous mutants at the 1781 position. Our study established the first case of fenoxaprop resistance in shortawn foxtail, determined cross resistance to other herbicides, and elucidated that the molecular basis of resistance resulted from, at least partly, an Ile to Leu mutation at amino acid position 1781 in the plastid ACCase.