Endothelial precursor cells in the peripheral blood of pregnant women
Endothelial precursor cells in the peripheral blood of pregnant women
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DOI：
10.1016/s1071-5576(02)00188-0
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发表时间：
2002-11-01
期刊：
影响因子：
--
通讯作者：
Elias, S
中科院分区：
文献类型：
--
作者：
Gussin, HAE;Bischoff, FZ;Elias, S
OBJECTIVE: To determine whether primitive endothelial precursor cells are present in the peripheral blood of pregnant compared with nonpregnant subjects and whether these precursor cells art, of fetal or maternal origin.METHODS: Peripheral blood mononuclear cells from 13 pregnant women ill the second trimester and from ten non pregnant women and men were cultured for 8-10 weeks under conditions that promoted endothelial cell development. Early outgrowth (1 week culture) and late outgrowth (4-6 weeks) colonies were observed, their endothelial nature was investigated, and fluorescence in situ hybridization was performed to determine tire origin of the colonies from pregnant women's specimens.RESULTS: Peripheral blood mononuclear cell cultures from all pregnant women and all nonpregnant controls yielded early-outgrowth endothelial cells. Late-outgrowth endothelial cells were observed in 61.5% (eight of 13) of pregnant subjects, but in none of the ten nonpregnant controls (chi(2) test; P < .01). The adherent cells stained positively for von Willebrand factor and incorporated Dil-Ac-LDL, confirming their endothelial origin. Fluorescence in situ hybridization analysis showed only X chromosome-specific signals and no Y chromosome-specific signals in the cells from the late-outgrowth endothelial cells in all pregnant women carrying either a male (n = 5) or a female (n = 8) fetus.CONCLUSION: Primitive endothelial precursor cells are present in most pregnant women during the second trimester. These cells appear to be of maternal origin. Copyright (C) 2002 by the Society for Gynecologic Investigation.
