Establishment and characterization of a new human testicular seminoma cell line, JKT-1.
Establishment and characterization of a new human testicular seminoma cell line, JKT-1.
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DOI：
10.1111/j.1442-2042.1998.tb00604.x
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发表时间：
1998-05-01
期刊：
影响因子：
--
通讯作者：
Tanaka, H
中科院分区：
文献类型：
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作者：
Kinugawa, K;Hyodo, F;Tanaka, H
背景：建立了一株新的人睾丸癌细胞株(JKT-1)，并成功地将其移植到裸鼠体内，至今已维持2年以上。我们检测了JKT-1细胞的生物学特性。方法：JKT-1的原始材料来自于一位40岁男性的左侧睾丸精原细胞瘤(纯型，典型)的原发病变。肿瘤组织切块，按组织块培养方法进行培养。结果：JKT-1细胞不产生甲胎蛋白和人绒毛膜促性腺激素，但对波形蛋白和胎盘碱性磷酸酶有较强的免疫反应性。染色体分析显示，该基因的模式数为66，但丢失了Y染色体。在形态上，JKT-1细胞呈多形性多角形，核浆比增大，细胞器和桥粒样细胞-细胞连接发育不良。将JKT-1细胞移植到6周龄裸鼠背部，生长出典型的精原细胞瘤组织。结论：本研究建立了一种可用于体外和体内实验系统的精原细胞瘤细胞系。未来的研究计划使用这种精原细胞瘤系来研究生殖细胞。
BACKGROUND: A new human testicular cancer cell line (JKT-1) was established, successfully transplanted into nude mice, and has been maintained for over 2 years. We examined the biological characteristics of JKT-1 cells.METHODS: The original material for JKT-1 was derived from a primary lesion of a left testicular seminoma (pure, typical-type) from a 40-year-old male. The tumor tissue was minced and cultured according to the explant culture method. The cells grew as a monolayer with a doubling time of 28.5 hours.RESULTS: JKT-1 produced neither alpha-fetoprotein or beta-human chorionic gonadotropin, but showed strong immunoreactivities for vimentin and placental alkaline phosphatase. A chromosomal analysis revealed a modal number of 66 with loss of the Y chromosome. Morphologically, JKT-1 cells have a pleomorphic polygonal shape, an increase in the nuclear/cytoplasm ratio, and poor development of organelle and desmosome-like cell-cell junctions. JKT-1 cells were subcutaneously transplanted into the backs of 6-week-old nude mice, and grew classical seminomatous tissue.CONCLUSION: This report profiled a seminoma cell line established for both in vitro and in vivo experimental systems. Future studies are planned to investigate germ cells using this seminoma line.