Myricitrin exhibits anti-atherosclerotic and anti-hyperlipidemic effects in diet-induced hypercholesterolemic rats
Myricitrin exhibits anti-atherosclerotic and anti-hyperlipidemic effects in diet-induced hypercholesterolemic rats
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DOI：
10.1186/s13568-019-0924-0
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发表时间：
2019-12-21
期刊：
影响因子：
3.7
通讯作者：
Wang, Rongmei
中科院分区：
文献类型：
--
作者：
Gao, Jing;Liu, Cuicui;Wang, Rongmei
本研究探讨了杨梅三醇对高胆固醇血症大鼠的抗动脉粥样硬化作用。将大鼠分为假药组（标准食品）、对照组[1%高胆固醇饮食（HCD）]、1 μ M杨梅三酯+ 1% HCD、10 μ M杨梅三酯+ 1% HCD、100 μ M杨梅三酯+ 1% HCD和阳性对照组（10 mg/kg体重阿托伐他汀）。大鼠连续灌胃45天。给大鼠喂食1%的HCD，导致低密度脂蛋白、胆固醇和甘油三酯（TG）水平显著增加，而高密度脂蛋白（HDL）水平降低。然而，补充杨梅三醇的大鼠胆固醇、低密度脂蛋白和甘油三酯水平降至接近正常水平，而高密度脂蛋白水平升高。与假药大鼠相比，hcd喂养的大鼠过氧化氢酶、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（Gpx）和还原性谷胱甘肽（GSH）水平显著降低。然而，添加100 μ M杨桃苷的大鼠，这些水平增加了约50%。杨梅三醇处理后，脂质过氧化和活性氧（ROS）水平降低。hcd喂养大鼠主动脉壁面积显著增加14.5%。然而，添加1、10和100 μ M杨梅三醇的大鼠主动脉壁面积分别显著减少2.3%、4%和27.5%。这是首次报道杨梅三酯对高胆固醇血症大鼠的抗动脉粥样硬化和降血脂作用。杨梅三醇降低高胆固醇血症大鼠血清总胆固醇水平及对主动脉粥样硬化的作用。
The present study investigated the anti-atherosclerotic potential of myricitrin in hypercholesterolemic rats. Rats were divided into the following groups: sham (standard food), control [1% high-cholesterol diet (HCD)], 1 mu M myricitrin + 1% HCD, 10 mu M myricitrin + 1% HCD, 100 mu M myricitrin + 1% HCD, and the positive control (10 mg/kg body weight atorvastatin). The dose was given to rats via oral gavage for 45 consecutive days. Feeding of rats with 1% HCD caused substantial increases in the levels of LDL, cholesterol, and triglycerides (TG), while high-density lipoprotein (HDL) was reduced. However, rats supplemented with myricitrin had reduced levels of cholesterol, LDL, and TG to near-normal levels, whereas HDL was increased. Catalase, superoxide dismutase (SOD), glutathione peroxidase (Gpx), and reduced glutathione (GSH) levels were substantially reduced in the HCD-fed rats compared with sham rats. However, the rats supplemented with 100 mu M myricitrin showed > 50% increases in these levels. Lipid peroxidation and reactive oxygen species (ROS) levels were reduced following myricitrin treatment. The aortic cell wall area was significantly increased by 14.5% in HCD-fed rats. However, rats supplemented with 1, 10, and 100 mu M myricitrin showed significant reductions in the aortic cell wall area of 2.3%, 4%, and 27.5%, respectively. This is the first report of the anti-atherosclerotic and hypolipidemic effects of myricitrin in hypercholesterolemic rats. Myricitrin decreased the level of total serum cholesterol and the role of aortic atherosclerosis in hypercholesterolemic rats.