Mitochondrial signal transduction in accelerated wound and retinal healing by near-infrared light therapy
Mitochondrial signal transduction in accelerated wound and retinal healing by near-infrared light therapy
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DOI：
10.1016/j.mito.2004.07.033
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发表时间：
2004-09-01
期刊：
影响因子：
4.4
通讯作者：
Whelan, HT
中科院分区：
文献类型：
--
作者：
Eells, JT;Wong-Riley, MTT;Whelan, HT
使用低能量激光器或发光二极管（LED）阵列通过红光至近红外范围（630-1000 nm）的光进行的光生物调节已被证明可加速伤口愈合、改善心脏缺血性损伤的恢复并减弱受损视神经的变性。最近的证据表明，红光至近红外光的治疗效果部分来自于由NIR光与线粒体光受体分子细胞色素c氧化酶的相互作用触发的细胞内信号传导机制。我们已经证明，NIR-LED光照射增加了细胞色素氧化酶在培养的原代神经元的生产和逆转代谢抑制剂产生的细胞色素氧化酶活性的降低。我们还表明，NIR-LED治疗可以预防儿童骨髓移植患者口腔粘膜炎的发展。光生物调节通过上调促进伤口愈合的重要基因来改善遗传性糖尿病小鼠的伤口愈合。最近的研究已经提供了证据，证明NIR-LED治疗在由线粒体毒素、甲醇中毒过程中产生的甲酸造成的急性损伤后，在体内视网膜和视神经的存活和功能恢复方面具有治疗益处。使用微阵列技术进行的基因发现研究记录了参与线粒体能量产生和抗氧化细胞保护的途径中的基因表达的显著上调。这些发现提供了红光至近红外光对体外线粒体氧化代谢和体内细胞损伤的作用之间的联系。基于这些发现和线粒体功能障碍参与许多疾病过程的发病机制的强有力证据，我们提出NIR-LED光生物调节代表了用于治疗组织损伤和疾病过程的创新和非侵入性治疗方法，其中线粒体功能障碍被假定发挥作用，包括糖尿病视网膜病变，年龄相关性黄斑变性，Leber遗传性视神经病变与帕金森病(C)2004年Elsevier B. V.和线粒体研究学会。All rights reserved.
Photobiomodulation by light in the red to near infrared range (630-1000 nm) using low energy lasers or light-emitting diode (LED) arrays has been shown to accelerate wound healing, improve recovery from ischemic injury in the heart and attenuate degeneration in the injured optic nerve. Recent evidence indicates that the therapeutic effects of red to near infrared light result, in part, from intracellular signaling mechanisms triggered by the interaction of NIR light with the mitochondrial photoacceptor molecule cytochrome c oxidase. We have demonstrated that NIR-LED photo-irradiation increases the production of cytochrome oxidase in cultured primary neurons and reverses the reduction of cytochrome oxidase activity produced by metabolic inhibitors. We have also shown that NIR-LED treatment prevents the development of oral mucositis in pediatric bone marrow transplant patients. Photobiomodulation improves wound healing in genetically diabetic mice by upregulating genes important in the promotion of wound healing. More recent studies have provided evidence for the therapeutic benefit of NIR-LED treatment in the survival and functional recovery of the retina and optic nerve in vivo after acute injury by the mitochondrial toxin, formic acid generated in the course of methanol intoxication. Gene discovery studies conducted using microarray technology documented a significant upregulation of gene expression in pathways involved in mitochondrial energy production and antioxidant cellular protection. These findings provide a link between the actions of red to near infrared light on mitochondrial oxidative metabolism in vitro and cell injury in vivo. Based on these findings and the strong evidence that mitochondrial dysfunction is involved in the pathogenesis of numerous diseases processes, we propose that NIR-LED photobiomodulation represents an innovative and non-invasive therapeutic approach for the treatment of tissue injury and disease processes in which mitochondrial dysfunction is postulated to play a role including diabetic retinopathy, age-related macular degeneration, Leber's hereditary optic neuropathy and Parkinson's disease. (C) 2004 Elsevier B.V. and Mitochondria Research Society. All rights reserved.