[Penetrative needling improves neurological function by up-regulating expression of autophagy related protein LC3 in rats with hemorrhagic stroke].
[Penetrative needling improves neurological function by up-regulating expression of autophagy related protein LC3 in rats with hemorrhagic stroke].
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DOI：
10.13702/j.1000-0607.180860
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发表时间：
2019-09-25
期刊：
影响因子：
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通讯作者：
Sun, Xiao-Wei
中科院分区：
文献类型：
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作者：
Liu, Hao;Zhang, Beng;Sun, Xiao-Wei
目的：观察透刺“百会”“渠滨”穴对出血性脑卒中大鼠神经功能及自噬相关蛋白微管相关蛋白轻链3（LC 3）表达的影响，探讨透刺“百会”“渠滨”穴改善出血性脑卒中的作用机制。将120只雄性SD大鼠随机分为假手术组、模型组、非穴位组、针刺组和药物（雷帕霉素）组，再分为造模后3、7 d两个时间点亚组，每组12只。通过将大鼠自血（50穆尔）注入壳核区建立脑出血模型。从GV 20到GB 7或假穴（GV 20和GB 7旁约1cm）透刺30 min，每日2次，连续7 d。给药组大鼠右侧脑室注射雷帕霉素溶液（7 μ mol/L）。神经功能（运动和平衡功能障碍）采用悬吊钢丝试验（0-6分）和水平板行走试验（0-6分）进行评价。采用免疫组化和Western blot方法分别检测缺血半暗带区脑组织总LC 3的表达和LC 3-Ⅰ、LC 3-Ⅱ蛋白的表达。造模后第3、7天神经功能评分明显下降，与假手术组相比，模型组LC 3-Ⅱ/Ⅰ和LC 3蛋白在第3、7天均显著升高（P
OBJECTIVE: To observe the effect of penetrative needling from "Baihui" (GV20) to "Qubin" (GB7) on neurological function and expression of autophagy related protein microtubule-associated protein, light chain 3 (LC3) in rats with hemorrhagic stroke, so as to explore its mechanism underlying improvement of hemorrhagic stroke.METHODS: A total of 120 male SD rats were randomly divided into sham operation, model, non-acupoint, acupuncture and medication (Rapamycin) groups which were further divided into two time-point subgroups (3, 7 days after modeling, n=12/subgroup). The intracerebral hemorrhage model was established by injection of the rat's auto-blood (50 muL) into the putaman region. Penetrative needling from GV20 to GB7 or sham acupoints (about 1 cm beside GV20 and GB7) was conducted for 30 min, twice daily for 7 days. For rats of the medication group, Rapamycin solution (7 mumol/L) was injected into the right lateral ventricle. The neurological functions (locomotor and balance deficits) were evaluated according to suspended wire test (0-6 points) and horizontal board walking test (0-6 points). Immunohistochemistry and Western blot were used to detect the expression of total LC3 and expression of LC3-Ⅰ and LC3-Ⅱ proteins in the ischemic penumbra region of brain tissue, respectively.RESULTS: After modeling, the neurological function scores were significantly decreased on day 3 and 7, the levels of LC3-Ⅱ/Ⅰ and LC3 protein on day 3 and 7 were significantly increased in the model group relevant to the sham operation group (P