In vitro and in vivo two-photon luminescence imaging of single gold nanorods
In vitro and in vivo two-photon luminescence imaging of single gold nanorods
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DOI：
10.1073/pnas.0504892102
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发表时间：
2005-11-01
影响因子：
11.1
通讯作者：
Cheng, JX
中科院分区：
文献类型：
--
作者：
Wang, HF;Huff, TB;Cheng, JX
在远场激光扫描显微镜上激发的830 nm波长的金纳米棒产生了很强的双光子发光(TPL)，其发光强度与入射偏振态有关。TPL激发光谱可以叠加到纵向等离子体激元带上，表明等离子体激元增强的双光子吸收截面。单个纳米棒的TPL信号是单个罗丹明分子双光子荧光信号的58倍。通过对单个纳米棒在小鼠耳血管中流动的活体成像，证明了金纳米棒作为第三方荧光显像剂的应用。
Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos(4) dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.