Strong Wavelength Dependence of Hyperpolarizability in the Near-Infrared Biological Window for Second-Harmonic Generation by Amphiphilic Porphyrins
Strong Wavelength Dependence of Hyperpolarizability in the Near-Infrared Biological Window for Second-Harmonic Generation by Amphiphilic Porphyrins
复制标题
DOI：
10.1021/jp302050j
复制
发表时间：
2012-06-28
影响因子：
3.7
通讯作者：
Clays, Koen
中科院分区：
文献类型：
--
作者：
De Mey, Kurt;Perez-Moreno, Javier;Clays, Koen
我们已经应用的Tumblas-Kuhn求和规则来建模的二阶非线性极化率的波长依赖性的两亲性卟啉生色团设计用于细胞成像的基础上，其线性吸收光谱的完整分析。我们预测巨大的振荡，这第一超极化率在生物透明窗口与二阶响应表现出三个极小值和两个极大值在700和900 nm之间的波长范围和第二区域之间的增强响应1200和1500 nm。我们通过实验证实了预测值，证明了我们方法的有效性和波长扫描的必要性，以找到用于细胞成像的共振增强信号的最大值。这些结果提出了一种新的方法，实现光谱选择性在二次谐波产生成像。
We have applied the Thomas-Kuhn sum rules to model the wavelength dependence of the second-order nonlinear polarizability of an amphiphilic porphyrin chromophore designed for cellular imaging on the basis of the complete analysis of its linear absorption spectrum. We predict huge oscillations for this first hyperpolarizability in the biological transparency window with the second-order response exhibiting three minima and two maxima in the wavelength range between 700 and 900 nm and a second region of enhanced response between 1200 and 1500 nm. We confirmed the predicted values experimentally demonstrating both the validity of our approach and the need for a wavelength scan to find a maximum in the resonance-enhanced signal for cellular imaging. These results suggest a new approach toward achieving spectroscopic selectivity during second-harmonic generation imaging.