INITIAL INTRAMEMBRANEOUS OSTEOGENESIS INVITRO
INITIAL INTRAMEMBRANEOUS OSTEOGENESIS INVITRO
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DOI：
10.1002/aja.1001490403
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发表时间：
1977-01-01
影响因子：
--
通讯作者：
BERNARD, GW
中科院分区：
文献类型：
--
作者：
MARVASO, V;BERNARD, GW
将子宫内12-13天的胎鼠颅骨置于Rose小室中并在补充有20%胎牛血清和抗生素的BGJb培养基中培养。 7天和12天后，收获外植体并进行光学显微镜和电子显微镜检查。经过短暂的滞后时间后，间充质细胞分化成成骨细胞，从而产生编织骨。在体外和体内，生长和发育具有初始膜内成骨的所有相同特征：间充质细胞迁移和分化为成骨细胞，随后在细胞外空间中出现基质囊泡，这些囊泡内部和周围的羟基磷灰石结晶，羟基磷灰石晶体生长成骨的球形结节，以及随后这些结节融合成编织骨的接缝。在体外分化为成骨细胞和初始钙化的系统中观察到细胞参与初始成骨，这表明导致这些现象的事件已经在细胞形态分化之前发生在细胞内。
Calvariae of fetal mice 12-13 days in utero were placed in Rose chambers and cultured in BGJb medium supplemented with 20% fetal calf serum and antibiotics. After periods of 7 and 12 days the explants were harvested and processed for light microscopy and EM. The mesenchymal cells, after a brief lag time, differentiated into osteoblasts which produced woven bone. In vitro and in vivo, growth and development shared all of the same characteristics of initial intramembraneous osteogenesis: migration and differentiation of mesenchymal cells into osteoblasts, the subsequent appearance of matrix vesicles in the extracellular space, crystallization of hydroxyapatite within and about these vesicles, growth of hydroxyapatite crystals into spheroidal nodules of bone and the subsequent fusion of these nodules into seams of woven bone. Cellular involvement in initial osteogenesis was observed in a system where differentiation into osteoblasts and initial calcification took place in vitro, suggesting that the events responsible for these phenomena have occurred within the cells prior to their morphological differentiation.